RRC ID 48179
Author Akter S, Park JH, Yin CS.
Title Microbacterium horti sp. nov., a bacterium isolated from Cucurbita maxima cultivating soil.
Journal Arch Microbiol
Abstract A novel bacterial strain THG-SL1(T) was isolated from a soil sample of Cucurbita maxima garden and was characterized by using a polyphasic approach. Cells were Gram-reaction-positive, non-motile and rod-shaped. The strain was aerobic, catalase positive and weakly positive for oxidase. Phylogenetic analysis based on 16S rRNA gene sequence analysis but it shared highest similarity with Microbacterium ginsengisoli KCTC 19189(T) (96.6 %), indicating that strain THG-SL1(T) belongs to the genus Microbacterium. The DNA G + C content of the isolate was 68.9 mol %. The major fatty acids were anteiso-C15: 0 (39.7 %), anteiso-C17: 0 (24.4 %) and iso-C16: 0 (18.5 %). The major polar lipids of strain THG-SL1(T) were phosphatidylglycerol (PG) and an unidentified glycolipid (GL). The predominant respiratory isoprenoid quinones were menaquinone-11 and menaquinone-12. The diamino acid in the cell-wall peptidoglycan was ornithine. Based on the results of polyphasic characterization, strain THG-SL1(T) represented a novel species within the genus Microbacterium, for which the name Microbacterium horti sp. nov. is proposed. The type strain is THG-SL1(T) (=KACC 18286(T)=CCTCC AB 2015117(T)).
Volume 198(3)
Pages 233-40
Published 2016-4-1
DOI 10.1007/s00203-015-1183-3
PII 10.1007/s00203-015-1183-3
PMID 26757723
MeSH Actinomycetales / chemistry Actinomycetales / classification* Actinomycetales / genetics Actinomycetales / metabolism Base Composition Cucurbita Fatty Acids / analysis Molecular Sequence Data Peptidoglycan / metabolism Phylogeny* RNA, Ribosomal, 16S / genetics Soil Microbiology* Species Specificity Vitamin K 2 / metabolism
IF 1.884
Times Cited 1
WOS Category MICROBIOLOGY
Resource
General Microbes JCM 14902