論文 - 詳細
| RRC ID | 48197 |
|---|---|
| 著者 | Bharathiraja B, Ranjith Kumar R, PraveenKumar R, Chakravarthy M, Yogendran D, Jayamuthunagai J. |
| タイトル | Biodiesel production from different algal oil using immobilized pure lipase and tailor made rPichia pastoris with Cal A and Cal B genes. |
| ジャーナル | Bioresour Technol |
| Abstract |
In this investigation, oil extraction was performed in marine macroalgae Gracilaria edulis, Enteromorpha compressa and Ulva lactuca. The algal biomass was characterized by Scanning Electron Microscopy and Fourier Transform-Infra Red Spectroscopy. Six different pre-treatment methods were carried out to evaluate the best method for maximum oil extraction. Optimization of extraction parameters were performed and high oil yield was obtained at temperature 55°C, time 150min, particle size 0.10mm, solvent-to-solid ratio 6:1 and agitation rate 500rpm. After optimization, 9.5%, 12.18% and 10.50 (g/g) of oil extraction yield was achieved from the respective algal biomass. The rate constant for extraction was obtained as first order kinetics, by differential method. Stable intracellular Cal A and Cal B lipase producing recombinant Pichia pastoris was constructed and used as biocatalyst for biodiesel production. Comparative analysis of lipase activity and biodiesel yield was made with immobilized Candida antarctica lipase. |
| 巻・号 | 213 |
| ページ | 69-78 |
| 公開日 | 2016-8-1 |
| DOI | 10.1016/j.biortech.2016.02.041 |
| PII | S0960-8524(16)30167-5 |
| PMID | 26906444 |
| MeSH | Biocatalysis Biofuels* / analysis Biotechnology / methods* Cooking Enzymes, Immobilized / metabolism* Esterification Fatty Acids / analysis Kinetics Lipase / metabolism* Oils / metabolism* Pichia / metabolism* Seaweed / metabolism* Spectroscopy, Fourier Transform Infrared Ulva / metabolism Waste Products |
| IF | 7.539 |
| 引用数 | 12 |
| WOS 分野 | AGRICULTURAL ENGINEERING BIOTECHNOLOGY & APPLIED MICROBIOLOGY ENERGY & FUELS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 10318 |