RRC ID 48205
Author Ehara T, Izumi H, Tsuda M, Nakazato Y, Iwamoto H, Namba K, Takeda Y.
Title Combinational effects of prebiotic oligosaccharides on bifidobacterial growth and host gene expression in a simplified mixed culture model and neonatal mice.
Journal Br J Nutr
Abstract It is important to provide formula-fed infants with a bifidobacteria-enriched gut microbiota similar to those of breastfed infants to ensure intestinal health. Prebiotics, such as certain oligosaccharides, are a useful solution to this problem, but the combinational benefits of these oligosaccharides have not been evaluated. This study investigated the benefits of oligosaccharide combinations and screened for an optimal combination of oligosaccharides to promote healthy gut microbiota of formula-fed infants. In vitro and in vivo experiments were performed to assess the bifidogenic effects of lactulose (LAC) alone and LAC combined with raffinose (RAF) and/or galacto-oligosaccharide (GOS), using a mixed culture model and neonatal mice orally administered with these oligosaccharides and Bifidobacterium breve. In the in vitro culture model, the combination of the three oligosaccharides (LAC-RAF-GOS) significantly increased cell numbers of B. breve and Bifidobacterium longum (P<0·05) compared with either LAC alone or the combination of two oligosaccharides, and resulted in the production of SCFA under anaerobic conditions. In the in vivo experiment, the LAC-RAF-GOS combination significantly increased cell numbers of B. breve and Bacteroidetes in the large intestinal content (P<0·05) and increased acetate concentrations in the caecal content and serum of neonatal mice. Genes related to metabolism and immune responses were differentially expressed in the liver and large intestine of mice administered with LAC-RAF-GOS. These results indicate a synergistic effect of the LAC-RAF-GOS combination on the growth of bifidobacteria and reveal possible benefits of this combination to the gut microbiota and health of infants.
Volume 116(2)
Pages 270-8
Published 2016-7-1
DOI 10.1017/S0007114516001987
PII S0007114516001987
PMID 27198516
MeSH Animals Animals, Newborn Bifidobacterium / drug effects* Bifidobacterium / growth & development Breast Feeding Cecum / microbiology Fatty Acids, Volatile / metabolism Galactose / pharmacology Gastrointestinal Microbiome / drug effects* Gene Expression / drug effects* Humans Infant Formula / microbiology* Infant, Newborn Intestine, Large* / metabolism Intestine, Large* / microbiology Lactulose / pharmacology Liver / drug effects Liver / metabolism Male Mice, Inbred C57BL Oligosaccharides / pharmacology* Prebiotics* Raffinose / pharmacology
IF 3.334
Times Cited 13
General Microbes JCM 1217 JCM 1649 JCM 20624 JCM 5825 JCM 1131