| RRC ID |
48205
|
| 著者 |
Ehara T, Izumi H, Tsuda M, Nakazato Y, Iwamoto H, Namba K, Takeda Y.
|
| タイトル |
Combinational effects of prebiotic oligosaccharides on bifidobacterial growth and host gene expression in a simplified mixed culture model and neonatal mice.
|
| ジャーナル |
Br J Nutr
|
| Abstract |
It is important to provide formula-fed infants with a bifidobacteria-enriched gut microbiota similar to those of breastfed infants to ensure intestinal health. Prebiotics, such as certain oligosaccharides, are a useful solution to this problem, but the combinational benefits of these oligosaccharides have not been evaluated. This study investigated the benefits of oligosaccharide combinations and screened for an optimal combination of oligosaccharides to promote healthy gut microbiota of formula-fed infants. In vitro and in vivo experiments were performed to assess the bifidogenic effects of lactulose (LAC) alone and LAC combined with raffinose (RAF) and/or galacto-oligosaccharide (GOS), using a mixed culture model and neonatal mice orally administered with these oligosaccharides and Bifidobacterium breve. In the in vitro culture model, the combination of the three oligosaccharides (LAC-RAF-GOS) significantly increased cell numbers of B. breve and Bifidobacterium longum (P<0·05) compared with either LAC alone or the combination of two oligosaccharides, and resulted in the production of SCFA under anaerobic conditions. In the in vivo experiment, the LAC-RAF-GOS combination significantly increased cell numbers of B. breve and Bacteroidetes in the large intestinal content (P<0·05) and increased acetate concentrations in the caecal content and serum of neonatal mice. Genes related to metabolism and immune responses were differentially expressed in the liver and large intestine of mice administered with LAC-RAF-GOS. These results indicate a synergistic effect of the LAC-RAF-GOS combination on the growth of bifidobacteria and reveal possible benefits of this combination to the gut microbiota and health of infants.
|
| 巻・号 |
116(2)
|
| ページ |
270-8
|
| 公開日 |
2016-7-1
|
| DOI |
10.1017/S0007114516001987
|
| PII |
S0007114516001987
|
| PMID |
27198516
|
| MeSH |
Animals
Animals, Newborn
Bifidobacterium / drug effects*
Bifidobacterium / growth & development
Breast Feeding
Cecum / microbiology
Fatty Acids, Volatile / metabolism
Galactose / pharmacology
Gastrointestinal Microbiome / drug effects*
Gene Expression / drug effects*
Humans
Infant Formula / microbiology*
Infant, Newborn
Intestine, Large* / metabolism
Intestine, Large* / microbiology
Lactulose / pharmacology
Liver / drug effects
Liver / metabolism
Male
Mice, Inbred C57BL
Oligosaccharides / pharmacology*
Prebiotics*
Raffinose / pharmacology
|
| IF |
3.334
|
| 引用数 |
13
|
|
WOS 分野
|
NUTRITION & DIETETICS
|
| リソース情報 |
| 一般微生物 |
JCM 1217
JCM 1649
JCM 20624
JCM 5825
JCM 1131 |
