RRC ID 48460
著者 Lee JJ, Kang MS, Joo ES, Jung HY, Kim MK.
タイトル Hymenobacter sedentarius sp. nov., isolated from a soil.
ジャーナル J Microbiol
Abstract A novel Gram-negative and red-pinkish bacterium designated DG5B(T) was isolated from a dry soil. Cells were rods that were catalase- and oxidase-positive, and non-motile. The strain was found to grow at temperatures from 10 to 30°C (optimum 25°C) and pH 6.0-8.0, (optimum pH 7) on R2A broth. 16S rRNA gene sequence (1,452 bp) analysis of this strain identified it as a member of the genus Hymenobacter that belongs to the class Cytophagia. The highest gene sequence similarities were with Hymenobacter arizonensis OR362-8(T) (98.3%), Hymenobacter humi DG31A(T) (97.6%), and Hymenobacter glaciei VUG-A130(T) (96.6%). Strain DG5B(T) exhibited <70% DNA-DNA relatedness with H. arizonensis (34.7 ± 7.0%; reciprocally, 29.7 ± 1.2%) and H. humi (39.4 ± 4.3%; reciprocally, 39.5 ± 3.3%) as a different genomic species, and its genomic DNA G+C content was 59.8%. Strain DG5B(T) had the following chemotaxonomic characteristics: the major fatty acids are iso-C15:0, anteiso-C15:0, C16:1 ω5c, and summed feature 3 (C16:1 ω7c / C16:1 ω6c); polar lipid profile contained phosphatidylethanolamine (PE), unknown aminophospholipid (APL), unknown glycolipids (GL), unknown phospholipids (PL), and unknown polar lipids (L); the major quinone is MK-7. The absorbance peak of pigment is at 481.0 nm. Strain DG5B(T) showed low-level resistance to gamma-ray irradiation. Phenotypic, chemotaxonomic, and genotypic properties indicated that isolate DG5B(T) represents a novel species within the genus Hymenobacter for which the name Hymenobacter sedentarius sp. nov. is proposed. The type strain is DG5B(T) (=KCTC 32524(T) =JCM 19636(T)).
巻・号 54(4)
ページ 283-9
公開日 2016-4-1
DOI 10.1007/s12275-016-5386-3
PII 10.1007/s12275-016-5386-3
PMID 27033203
MeSH Bacteroidetes / classification* Bacteroidetes / cytology Bacteroidetes / isolation & purification* Bacteroidetes / physiology Base Composition DNA, Bacterial / chemistry DNA, Bacterial / genetics Gamma Rays Genes, rRNA Hydrogen-Ion Concentration Lipids / analysis Nucleic Acid Hybridization Phenotype Phylogeny RNA, Ribosomal, 16S / genetics Seoul Soil Microbiology* Temperature
IF 2.845
引用数 4
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 19636 JCM 13504 JCM 17225