論文 - 詳細
| RRC ID | 48508 |
|---|---|
| 著者 | Kim SY, Kim SH, Kim SN, Kim AR, Kim YR, Kim MJ, Park WS, Lee JH, Jung WH, Lee YW, Choe YB, Ahn KJ. |
| タイトル | Isolation and identification of Malassezia species from Chinese and Korean patients with seborrheic dermatitis and in vitro studies on their bioactivity on sebaceous lipids and IL-8 production. |
| ジャーナル | Mycoses |
| Abstract |
We investigated the distribution of Malassezia yeast in 120 Chinese (20 patients from each of six cities) and 20 Korean patients with scalp seborrheic dermatitis (SD) and dandruff (SD/D) using ITS1 and ITS2 polymerase chain reaction-restriction fragment length polymorphism. Bioactivity was studied by quantifying sebum lipid production by human primary sebocytes and inflammatory cytokine, interleukin-8 (IL-8) production was studied by exposing HaCaT keratinocytes with extracts of five standard Malassezia strains; M. globosa, M. restricta, M. sympodialis, M. dermatis and M. slooffiae. M. restricta and M. globosa were the most frequently encountered species from both Chinese and Korean patients. These two Malassezia species also promoted neutral lipid synthesis although the result was not statistically significant and induced significant increase in IL-8 production among the five Malassezia species studied. The study suggests a possible role of these organisms in the pathogenesis of SD/D. |
| 巻・号 | 59(5) |
| ページ | 274-80 |
| 公開日 | 2016-5-1 |
| DOI | 10.1111/myc.12456 |
| PMID | 26786542 |
| MeSH | Adult Aged Cells, Cultured China DNA, Fungal / isolation & purification DNA, Intergenic / analysis DNA, Ribosomal / analysis Dandruff / microbiology Dermatitis, Seborrheic / microbiology* Female Genome, Fungal / genetics Humans Interleukin-8 / biosynthesis* Keratinocytes / cytology Lipids / biosynthesis Malassezia / classification Malassezia / genetics Malassezia / immunology Malassezia / isolation & purification* Middle Aged Polymerase Chain Reaction Polymorphism, Restriction Fragment Length RNA, Ribosomal / genetics Scalp Dermatoses / microbiology* Sebum / metabolism* Seoul Urban Population |
| IF | 3.575 |
| 引用数 | 4 |
| WOS 分野 | DERMATOLOGY MYCOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 11348 |