| RRC ID |
48529
|
| Author |
Sangal V, Goodfellow M, Jones AL, Schwalbe EC, Blom J, Hoskisson PA, Sutcliffe IC.
|
| Title |
Next-generation systematics: An innovative approach to resolve the structure of complex prokaryotic taxa.
|
| Journal |
Sci Rep
|
| Abstract |
Prokaryotic systematics provides the fundamental framework for microbiological research but remains a discipline that relies on a labour- and time-intensive polyphasic taxonomic approach, including DNA-DNA hybridization, variation in 16S rRNA gene sequence and phenotypic characteristics. These techniques suffer from poor resolution in distinguishing between closely related species and often result in misclassification and misidentification of strains. Moreover, guidelines are unclear for the delineation of bacterial genera. Here, we have applied an innovative phylogenetic and taxogenomic approach to a heterogeneous actinobacterial taxon, Rhodococcus, to identify boundaries for intrageneric and supraspecific classification. Seven species-groups were identified within the genus Rhodococcus that are as distantly related to one another as they are to representatives of other mycolic acid containing actinobacteria and can thus be equated with the rank of genus. It was also evident that strains assigned to rhodococcal species-groups are underspeciated with many misclassified using conventional taxonomic criteria. The phylogenetic and taxogenomic methods used in this study provide data of theoretical value for the circumscription of generic and species boundaries and are also of practical significance as they provide a robust basis for the classification and identification of rhodococci of agricultural, industrial and medical/veterinary significance.
|
| Volume |
6
|
| Pages |
38392
|
| Published |
2016-12-7
|
| DOI |
10.1038/srep38392
|
| PII |
srep38392
|
| PMID |
27924912
|
| PMC |
PMC5141411
|
| MeSH |
Bacterial Typing Techniques / methods*
DNA Barcoding, Taxonomic / methods*
DNA, Bacterial / genetics*
Genome, Bacterial*
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Rhodococcus / classification
Rhodococcus / genetics*
Sequence Analysis, DNA
Species Specificity
Whole Genome Sequencing
|
| IF |
3.998
|
| Times Cited |
44
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 15477 |
