RRC ID |
48536
|
著者 |
Sanglas A, Albarral V, Farfán M, Lorén JG, Fusté MC.
|
タイトル |
Direct evidence of recombination in the recA gene of Aeromonas bestiarum.
|
ジャーナル |
Syst Appl Microbiol
|
Abstract |
Two hundred and twenty-one strains representative of all Aeromonas species were characterized using the recA gene sequence, assessing its potential as a molecular marker for the genus Aeromonas. The inter-species distance values obtained demonstrated that recA has a high discriminatory power. Phylogenetic analysis, based on full-length gene nucleotide sequences, revealed a robust topology with clearly separated clusters for each species. The maximum likelihood tree showed the Aeromonas bestiarum strains in a well-defined cluster, containing a subset of four strains of different geographical origins in a deep internal branch. Data analysis provided strong evidence of recombination at the end of the recA sequences in these four strains. Intergenomic recombination corresponding to partial regions of the two adjacent genes recA and recX (248 bp) was identified between A. bestiarum (major parent) and Aeromonas eucrenophila (minor parent). The low number of recombinant strains detected (1.8%) suggests that horizontal flow between recA sequences is relatively uncommon in this genus. Moreover, only a few nucleotide differences were detected among these fragments, indicating that recombination has occurred recently. Finally, we also determined if the recombinant fragment could have influenced the structure and basic functions of the RecA protein, comparing models reconstructed from the translated amino acid sequences of our A. bestiarum strains with known Escherichia coli RecA structures.
|
巻・号 |
39(2)
|
ページ |
106-14
|
公開日 |
2016-3-1
|
DOI |
10.1016/j.syapm.2016.01.003
|
PII |
S0723-2020(16)00017-5
|
PMID |
26934994
|
MeSH |
Aeromonas / genetics*
Genes, Bacterial
Models, Molecular
Phylogeny
Protein Conformation
Rec A Recombinases / chemistry
Rec A Recombinases / genetics*
Recombination, Genetic*
Sequence Analysis, DNA
|
IF |
3.224
|
引用数 |
4
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 3967
JCM 3968
JCM 3976
JCM 3995 |