Reference - Detail
| RRC ID | 48558 |
|---|---|
| Author | Fukuda M, Mikoshiba K. |
| Title | Characterization of KIAA1427 protein as an atypical synaptotagmin (Syt XIII). |
| Journal | Biochem J |
| Abstract |
Synaptotagmin (Syt) belongs to a family of type-I membrane proteins and is a protein that consists of a short extracellular N-terminus, a single transmembrane domain, two C2 domains and a short C-terminus. Here, we cloned and characterized a mouse orthologue of human KIAA1427 protein as an atypical Syt (named Syt XIII). Subcellular fractionation and antibody-uptake experiments indicate that Syt XIII is indeed a type-I membrane protein, but, unlike other Syt isoforms, lacks an N-terminal extracellular domain. Syt XIII C2 domains show relatively little similarity to Syt I (less than 35% identity at the amino acid level), and lack key amino acids responsible for Ca2+ binding. Because of these substitutions, the Syt XIII C2 domains did not show Ca2+-dependent phospholipid-binding activity, and Syt XIII is thus classified as a Ca2+ -independent isoform. By contrast, the Syt XIII C-terminal domain is highly homologous with other Syt isoforms and can function as a common receptor for neurexin Ialpha in vitro. Since Syt XIII is expressed in various tissues outside the brain, Syt XIII may be involved in constitutive vesicle transport. |
| Volume | 354(Pt 2) |
| Pages | 249-57 |
| Published | 2001-3-1 |
| DOI | 10.1042/0264-6021:3540249 |
| PMID | 11171101 |
| PMC | PMC1221650 |
| MeSH | Amino Acid Sequence Animals Base Sequence COS Cells Calcium-Binding Proteins* Cloning, Molecular DNA, Complementary / chemistry Electrophoresis, Polyacrylamide Gel Glycoproteins Humans Membrane Glycoproteins / chemistry* Membrane Glycoproteins / genetics Mice Molecular Sequence Data Nerve Tissue Proteins / chemistry* Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neuropeptides Phospholipids / metabolism Phylogeny Polymers / metabolism Protein Conformation RNA, Messenger / analysis Receptors, Cell Surface / chemistry* Receptors, Cell Surface / genetics Sequence Alignment Synaptotagmins |
| IF | 4.097 |
| Times Cited | 40 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pEF-T7-Syt XIII (Syt13) (RDB14693) pEF-T7-Syt XIV (Syt14) (RDB14694). |