| RRC ID |
4856
|
| 著者 |
Kanda M, Wada H, Fujiwara S.
|
| タイトル |
Epidermal expression of Hox1 is directly activated by retinoic acid in the Ciona intestinalis embryo.
|
| ジャーナル |
Dev Biol
|
| Abstract |
Hox genes play important roles in the specification of spatial identity during development of vertebrate embryos. Retinoic acid regulates the transcription of Hox genes in vertebrates. We identified an epidermal enhancer in the 5' flanking region of an ortholog of Hox1 (Ci-Hox1) in the ascidian Ciona intestinalis. This enhancer element drives the transcription of a lacZ reporter gene in the epidermis in the posterior trunk and the anterior tail region of tailbud-stage embryos. Inhibition of retinoic acid synthesis resulted in inactivation of the expression of the reporter gene. The enhancer contains a putative retinoic acid response element. When this element was mutagenized, the expression of the reporter gene disappeared from the epidermis. This sequence was also required for the response to exogenously administered retinoic acid. A heterodimeric nuclear receptor, consisting of the retinoic acid receptor and retinoid X receptor, bound to this sequence. These results indicate that retinoic acid directly activates the epidermal enhancer of Ci-Hox1. This is the first demonstration that retinoic acid is necessary for endogenous gene expression in ascidian embryos.
|
| 巻・号 |
335(2)
|
| ページ |
454-63
|
| 公開日 |
2009-11-15
|
| DOI |
10.1016/j.ydbio.2009.09.027
|
| PII |
S0012-1606(09)01212-3
|
| PMID |
19782671
|
| MeSH |
Animals
Base Sequence
Ciona intestinalis / embryology*
Conserved Sequence
DNA Primers
Electrophoretic Mobility Shift Assay
Electroporation
Embryo, Nonmammalian / drug effects*
Embryo, Nonmammalian / metabolism
Enhancer Elements, Genetic
Epidermis / drug effects*
Epidermis / metabolism
Genes, Homeobox*
Genes, Reporter
Molecular Sequence Data
Transgenes
Tretinoin / pharmacology*
|
| IF |
2.896
|
| 引用数 |
25
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| カタユウレイボヤ・(ニッポンウミシダ) |
Wild C.int |
