RRC ID 48560
著者 Fukuda M.
タイトル Molecular cloning, expression, and characterization of a novel class of synaptotagmin (Syt XIV) conserved from Drosophila to humans.
ジャーナル J Biochem
Abstract Synaptotagmins (Syts) represent a large family of putative membrane trafficking proteins found in various species from different phyla. In this study, I identified a novel class of Syt (named Syt XIV) conserved from Drosophila to humans and its highly related molecule, Strep14 (Syt XIV-related protein). Although both Syt XIV and Strep14 belong to the C-terminal-type (C-type) tandem C2 protein family, only Syt XIV has a single transmembrane domain at the N-terminus and a putative fatty-acylation site just downstream of the transmembrane domain. Biochemical analyses have indicated that Syt XIV is a Ca(2+)-independent Syt (e.g., Syts VIII, XII, and XIII) and that, like other Syt family proteins, it is capable of forming a Ca(2+)-independent oligomer. Unlike other Syt isoforms, however, expression of Syt XIV and Strep14 mRNA is highly restricted to mouse heart and testis and absent in the brain, where most other Syts are abundantly expressed, suggesting that Syt XIV and Strep14 may be involved in membrane trafficking in specific tissues outside the brain. I also identified all of the C-type tandem C2 proteins in humans, the mouse, the fruit fly, a nematode, a plant, and a yeast and discuss the molecular evolution of the C-type tandem C2 protein families, including the Syt family, the Syt-like protein (Slp) family, and the Doc2 family.
巻・号 133(5)
ページ 641-9
公開日 2003-5-1
DOI 10.1093/jb/mvg082
PMID 12801916
MeSH Amino Acid Sequence Animals Arabidopsis / genetics COS Cells Calcium-Binding Proteins* Cloning, Molecular Conserved Sequence Drosophila / genetics Gene Expression Humans Membrane Glycoproteins / biosynthesis Membrane Glycoproteins / chemistry Membrane Glycoproteins / genetics* Membrane Glycoproteins / metabolism* Mice Molecular Sequence Data Nerve Tissue Proteins / biosynthesis Nerve Tissue Proteins / chemistry Nerve Tissue Proteins / genetics* Nerve Tissue Proteins / metabolism* Phospholipids / metabolism Phylogeny Protein Isoforms Protein Structure, Tertiary Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Sequence Alignment Sequence Homology, Amino Acid Synaptotagmins
IF 2.476
引用数 45
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
遺伝子材料 pEF-T7-Syt XIV (Syt14) (RDB14694) pEF-T7-Strep14 (Syt16) (RDB14696).