| RRC ID |
48668
|
| 著者 |
Guo B, Lei C, Kobayashi H, Ito T, Yalikun Y, Jiang Y, Tanaka Y, Ozeki Y, Goda K.
|
| タイトル |
High-throughput, label-free, single-cell, microalgal lipid screening by machine-learning-equipped optofluidic time-stretch quantitative phase microscopy.
|
| ジャーナル |
Cytometry A
|
| Abstract |
The development of reliable, sustainable, and economical sources of alternative fuels to petroleum is required to tackle the global energy crisis. One such alternative is microalgal biofuel, which is expected to play a key role in reducing the detrimental effects of global warming as microalgae absorb atmospheric CO2 via photosynthesis. Unfortunately, conventional analytical methods only provide population-averaged lipid amounts and fail to characterize a diverse population of microalgal cells with single-cell resolution in a non-invasive and interference-free manner. Here high-throughput label-free single-cell screening of lipid-producing microalgal cells with optofluidic time-stretch quantitative phase microscopy was demonstrated. In particular, Euglena gracilis, an attractive microalgal species that produces wax esters (suitable for biodiesel and aviation fuel after refinement), within lipid droplets was investigated. The optofluidic time-stretch quantitative phase microscope is based on an integration of a hydrodynamic-focusing microfluidic chip, an optical time-stretch quantitative phase microscope, and a digital image processor equipped with machine learning. As a result, it provides both the opacity and phase maps of every single cell at a high throughput of 10,000 cells/s, enabling accurate cell classification without the need for fluorescent staining. Specifically, the dataset was used to characterize heterogeneous populations of E. gracilis cells under two different culture conditions (nitrogen-sufficient and nitrogen-deficient) and achieve the cell classification with an error rate of only 2.15%. The method holds promise as an effective analytical tool for microalgae-based biofuel production. © 2017 International Society for Advancement of Cytometry.
|
| 巻・号 |
91(5)
|
| ページ |
494-502
|
| 公開日 |
2017-5-1
|
| DOI |
10.1002/cyto.a.23084
|
| PMID |
28399328
|
| MeSH |
Cell Count
High-Throughput Screening Assays / methods*
Machine Learning
Microalgae / cytology
Microfluidic Analytical Techniques / methods*
Microscopy / methods*
Single-Cell Analysis / methods*
|
| IF |
3.124
|
| 引用数 |
22
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
CELL BIOLOGY
|
| リソース情報 |
| 藻類 |
NIES-48 |
