RRC ID 48690
著者 Nakada T, Tomita M.
タイトル Morphology and phylogeny of a new wall-less freshwater volvocalean flagellate, Hapalochloris nozakii gen. et sp. nov. (Volvocales, Chlorophyceae).
ジャーナル J Phycol
Abstract New strains of a wall-less unicellular volvocalean flagellate were isolated from a freshwater environment in Japan. Observations of the alga, described here as Hapalochloris nozakii Nakada, gen. et sp. nov., were made using light, fluorescence, and electron microscopy. Each vegetative cell had two flagella, four contractile vacuoles, and a spirally furrowed cup-shaped chloroplast with an axial pyrenoid, and mitochondria located in the furrows. Based on the morphology, H. nozakii was distinguished from other known wall-less volvocalean flagellates. Under electron microscopy, fibrous material, instead of a cell wall and dense cortical microtubules, was observed outside and inside the cell membrane, respectively. Based on the phylogenetic analyses of 18S rRNA gene sequences, H. nozakii was found to be closely related to Asterococcus, Oogamochlamys, Rhysamphichloris, and "Dunaliella" lateralis and was separated from other known wall-less flagellate volvocaleans, indicating independent secondary loss of the cell wall in H. nozakii. In the combined 18S rRNA and chloroplast gene tree, H. nozakii was sister to Lobochlamys.
巻・号 53(1)
ページ 108-117
公開日 2017-2-1
DOI 10.1111/jpy.12484
PMID 27767210
MeSH Algal Proteins / genetics Amino Acid Sequence Japan Microscopy, Electron, Transmission Phylogeny* Sequence Alignment Species Specificity Volvocida / classification* Volvocida / cytology Volvocida / genetics Volvocida / ultrastructure*
IF 2.328
引用数 3
WOS 分野 MARINE & FRESHWATER BIOLOGY PLANT SCIENCES
リソース情報
藻類 NIES-2320 NIES-2254 NIES-3761 NIES-3762 NIES-3763 NIES-3705 NIES-3760