RRC ID 48752
著者 Deb BK, Pathak T, Hasan G.
タイトル Store-independent modulation of Ca(2+) entry through Orai by Septin 7.
ジャーナル Nat Commun
Abstract Orai channels are required for store-operated Ca(2+) entry (SOCE) in multiple cell types. Septins are a class of GTP-binding proteins that function as diffusion barriers in cells. Here we show that Septin 7 acts as a 'molecular brake' on activation of Orai channels in Drosophila neurons. Lowering Septin 7 levels results in dOrai-mediated Ca(2+) entry and higher cytosolic Ca(2+) in resting neurons. This Ca(2+) entry is independent of depletion of endoplasmic reticulum Ca(2+) stores and Ca(2+) release through the inositol-1,4,5-trisphosphate receptor. Importantly, store-independent Ca(2+) entry through Orai compensates for reduced SOCE in the Drosophila flight circuit. Moreover, overexpression of Septin 7 reduces both SOCE and flight duration, supporting its role as a negative regulator of Orai channel function in vivo. Septin 7 levels in neurons can, therefore, alter neural circuit function by modulating Orai function and Ca(2+) homeostasis.
巻・号 7
ページ 11751
公開日 2016-5-26
DOI 10.1038/ncomms11751
PII ncomms11751
PMID 27225060
PMC PMC4894974
MeSH Animals Calcium / metabolism* Calcium Signaling* Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Flight, Animal / physiology Gene Expression Regulation Inositol 1,4,5-Trisphosphate / metabolism Inositol 1,4,5-Trisphosphate Receptors / genetics Inositol 1,4,5-Trisphosphate Receptors / metabolism Larva / genetics Larva / growth & development Larva / metabolism Neurons / cytology Neurons / metabolism* ORAI1 Protein / genetics* ORAI1 Protein / metabolism Primary Cell Culture Protein Isoforms / genetics Protein Isoforms / metabolism Septins / genetics* Septins / metabolism
IF 12.121
引用数 16
リソース情報
ショウジョウバエ 1063R-1 1063R-2