RRC ID 48756
Author Tanaka-Hayashi A, Hayashi S, Inoue R, Ito T, Konno K, Yoshida T, Watanabe M, Yoshimura T, Mori H.
Title Is D-aspartate produced by glutamic-oxaloacetic transaminase-1 like 1 (Got1l1): a putative aspartate racemase?
Journal Amino Acids
Abstract D-Aspartate is an endogenous free amino acid in the brain, endocrine tissues, and exocrine tissues in mammals, and it plays several physiological roles. In the testis, D-aspartate is detected in elongate spermatids, Leydig cells, and Sertoli cells, and implicated in the synthesis and release of testosterone. In the hippocampus, D-aspartate strongly enhances N-methyl-D-aspartate receptor-dependent long-term potentiation and is involved in learning and memory. The existence of aspartate racemase, a candidate enzyme for D-aspartate production, has been suggested. Recently, mouse glutamic-oxaloacetic transaminase 1-like 1 (Got1l1) has been reported to synthesize substantially D-aspartate from L-aspartate and to be involved in adult neurogenesis. In this study, we investigated the function of Got1l1 in vivo by generating and analyzing Got1l1 knockout (KO) mice. We also examined the enzymatic activity of recombinant Got1l1 in vitro. We found that Got1l1 mRNA is highly expressed in the testis, but it is not detected in the brain and submandibular gland, where D-aspartate is abundant. The D-aspartate contents of wild-type and Got1l1 KO mice were not significantly different in the testis and hippocampus. The recombinant Got1l1 expressed in mammalian cells showed L-aspartate aminotransferase activity, but lacked aspartate racemase activity. These findings suggest that Got1l1 is not the major aspartate racemase and there might be an as yet unknown D-aspartate-synthesizing enzyme.
Volume 47(1)
Pages 79-86
Published 2015-1-1
DOI 10.1007/s00726-014-1847-3
PMID 25287256
PMC PMC4282708
MeSH Amino Acid Isomerases / chemistry Amino Acid Isomerases / genetics Amino Acid Isomerases / metabolism* Animals Brain / enzymology Brain / metabolism D-Aspartic Acid / metabolism* Female Hippocampus / enzymology Hippocampus / metabolism Male Mice Mice, Inbred C57BL Mice, Inbred ICR Mice, Knockout Testis / enzymology Testis / metabolism
IF 3.063
Times Cited 16
DNA material B6N BAC Mouse (RDB07573) B6Ng01-118F09