RRC ID |
48761
|
Author |
Wang L, Li Q, Zhang A, Zhou W, Jiang R, Yang Z, Yang H, Qin X, Ding S, Lu Q, Wen X, Lu C.
|
Title |
The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis.
|
Journal |
Mol Plant
|
Abstract |
Phytyl-diphosphate, which provides phytyl moieties as a common substrate in both tocopherol and phylloquinone biosynthesis, derives from de novo isoprenoid biosynthesis or a salvage pathway via phytol phosphorylation. However, very little is known about the role and origin of the phytyl moiety for phylloquinone biosynthesis. Since VTE6, a phytyl-phosphate kinase, is a key enzyme for phytol phosphorylation, we characterized Arabidopsis vte6 mutants to gain insight into the roles of phytyl moieties in phylloquinone biosynthesis and of phylloquinone in photosystem I (PSI) biogenesis. The VTE6 knockout mutants vte6-1 and vte6-2 lacked detectable phylloquinone, whereas the phylloquinone content in the VTE6 knockdown mutant vte6-3 was 90% lower than that in wild-type. In vte6 mutants, PSI function was impaired and accumulation of the PSI complex was defective. The PSI core subunits PsaA/B were efficiently synthesized and assembled into the PSI complex in vte6-3. However, the degradation rate of PSI subunits in the assembled PSI complex was more rapid in vte6-3 than in wild-type. In vte6-3, PSI was more susceptible to high-light damage than in wild-type. Our results provide the first genetic evidence that the phytol phosphorylation pathway is essential for phylloquinone biosynthesis, and that phylloquinone is required for PSI complex stability.
|
Volume |
10(1)
|
Pages |
183-196
|
Published |
2017-1-9
|
DOI |
10.1016/j.molp.2016.12.006
|
PII |
S1674-2052(16)30306-9
|
PMID |
28007557
|
MeSH |
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism
Gene Knockout Techniques
Light
Mutation
Phosphorylation
Phosphotransferases (Alcohol Group Acceptor) / genetics
Phosphotransferases (Alcohol Group Acceptor) / metabolism
Photosystem I Protein Complex / metabolism*
Photosystem I Protein Complex / radiation effects
Phytol / metabolism*
Protein Stability
Vitamin K 1 / metabolism*
|
IF |
12.084
|
Times Cited |
12
|
WOS Category
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
Resource |
Arabidopsis / Cultured plant cells, genes |
pst00121
pst15134 |