論文 - 詳細
| RRC ID | 48774 |
|---|---|
| 著者 | Kurita K, Sakamoto T, Yagi N, Sakamoto Y, Ito A, Nishino N, Sako K, Yoshida M, Kimura H, Seki M, Matsunaga S. |
| タイトル | Live imaging of H3K9 acetylation in plant cells. |
| ジャーナル | Sci Rep |
| Abstract |
Proper regulation of histone acetylation is important in development and cellular responses to environmental stimuli. However, the dynamics of histone acetylation at the single-cell level remains poorly understood. Here we established a transgenic plant cell line to track histone H3 lysine 9 acetylation (H3K9ac) with a modification-specific intracellular antibody (mintbody). The H3K9ac-specific mintbody fused to the enhanced green fluorescent protein (H3K9ac-mintbody-GFP) was introduced into tobacco BY-2 cells. We successfully demonstrated that H3K9ac-mintbody-GFP interacted with H3K9ac in vivo. The ratio of nuclear/cytoplasmic H3K9ac-mintbody-GFP detected in quantitative analysis reflected the endogenous H3K9ac levels. Under chemically induced hyperacetylation conditions with histone deacetylase inhibitors including trichostatin A, Ky-2 and Ky-14, significant enhancement of H3K9ac was detected by H3K9ac-mintbody-GFP dependent on the strength of inhibitors. Conversely, treatment with a histone acetyltransferase inhibitor, C646 caused a reduction in the nuclear to cytoplasmic ratio of H3K9ac-mintbody-GFP. Using this system, we assessed the environmental responses of H3K9ac and found that cold and salt stresses enhanced H3K9ac in tobacco BY-2 cells. In addition, a combination of H3K9ac-mintbody-GFP with 5-ethynyl-2'-deoxyuridine labelling confirmed that H3K9ac level is constant during interphase. |
| 巻・号 | 7 |
| ページ | 45894 |
| 公開日 | 2017-4-18 |
| DOI | 10.1038/srep45894 |
| PII | srep45894 |
| PMID | 28418019 |
| PMC | PMC5394682 |
| MeSH | Acetylation / drug effects Antibodies / immunology Antibodies / metabolism Benzoates / pharmacology Green Fluorescent Proteins / chemistry Histone Acetyltransferases / genetics* Histone Acetyltransferases / immunology Histone Acetyltransferases / ultrastructure Histone Deacetylase Inhibitors / pharmacology Histones / immunology Nitrobenzenes Plant Cells / metabolism Plant Cells / ultrastructure* Plants, Genetically Modified Protein Processing, Post-Translational / genetics* Pyrazoles / pharmacology Pyrazolones Tobacco / genetics Tobacco / growth & development Tobacco / metabolism* |
| IF | 3.998 |
| 引用数 | 3 |
| WOS 分野 | MULTIDISCIPLINARY SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00001 |