論文 - 詳細
| RRC ID | 48786 |
|---|---|
| 著者 | Makuta H, Obara K, Kihara A. |
| タイトル | Loop 5 region is important for the activity of the long-chain base transporter Rsb1. |
| ジャーナル | J Biochem |
| Abstract |
Intracellular lipid amounts are regulated not only by metabolism but also by efflux. Yeast Rsb1 is the only known transporter/floppase of the sphingolipid components long-chain bases (LCBs). However, even fundamental knowledge about Rsb1, such as important amino acid residues for activity and substrate recognition, still remains unclear. Rsb1 belongs to the Rta1-like family. To date, it has not been determined whether all family members share a common ability to export LCBs. Here, we revealed that within the Rta1-like family, only Rsb1 suppressed the hypersensitivity of the mutant cells lacking LCB 1-phoshate-degrading enzymes, suggesting that LCB-exporting activity is specific to Rsb1. Rsb1 contains a characteristic region (loop 5), which does not exist in other proteins of the Rta1-like family. We found that deletion of this region caused loss of Rsb1 function. Further mutational analysis of loop 5 revealed that the charged amino acid residues E223, D225 and R236 were important for Rsb1 activity. In addition to LCBs, Rsb1 facilitated the export of 1-hexadecanol, but not palmitic acid, which suggests that Rsb1 recognizes the C1 hydroxyl group. Thus, our findings provide an important clue for understanding the molecular mechanism of LCB export. |
| 巻・号 | 161(2) |
| ページ | 207-213 |
| 公開日 | 2017-2-1 |
| DOI | 10.1093/jb/mvw059 |
| PII | 2449696 |
| PMID | 28175317 |
| MeSH | Membrane Proteins / chemistry* Membrane Proteins / metabolism* Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / chemistry* Saccharomyces cerevisiae Proteins / metabolism* |
| IF | 2.476 |
| 引用数 | 1 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pHA_RSB1 (RDB15067) pHA_RTA1 (RDB15068) pHA_PUG1 (RDB15069) pHA_YLR046C (RDB15070) pHA_RSB1D100p5 (RDB15071) pRSB1-GFP (RDB15072). |