RRC ID 48788
Author Takahashi M, Buma Y, Iwamoto T, Inaguma Y, Ikeda H, Hiai H.
Title Cloning and expression of the ret proto-oncogene encoding a tyrosine kinase with two potential transmembrane domains.
Journal Oncogene
Abstract The nucleotide sequence of the ret proto-oncogene has been determined from cDNA clones isolated from a cDNA library of a THP-1 human monocytic leukemia cell line. Analysis of this sequence indicates that it encodes a protein which is structurally related to transmembrane receptors with a cytoplasmic tyrosine kinase domain. Unlike most growth factor receptors, it contains two hydrophobic regions which are potential transmembrane domains. Comparison of the sequence of the ret proto-oncogene with that of the ret transforming gene revealed that, in addition to the amino-terminal truncation, the last 51 carboxy-terminal amino acids of the ret proto-oncogene were replaced with 9 unrelated amino acids of the ret transforming gene. The focus forming activity of the ret cDNA, containing both amino-terminal and caboxy-terminal truncations, was approximately 13-fold higher than that of cDNA containing only the amino-terminal truncation. This suggests a possible role for the carboxy-terminal sequence in activation of transforming potential of the ret proto-oncogene. When transcription of the ret proto-oncogene was examined in a variety of mouse tissues, we detected its transcription in normal mouse spinal cord and lymphadenopathy of C3H/HeJ-gld/gld and MRL/Mp-lpr/lpr mice.
Volume 3(5)
Pages 571-8
Published 1988-11-1
PMID 3078962
MeSH Amino Acid Sequence Base Sequence Blotting, Northern Cell Line Cell Membrane / enzymology Cloning, Molecular DNA, Neoplasm / genetics Drosophila Proteins* Gene Expression Gene Library Humans Molecular Sequence Data Plasmids Protein Conformation Protein-Tyrosine Kinases / genetics* Proto-Oncogene Mas Proto-Oncogene Proteins / genetics* Proto-Oncogene Proteins c-ret Proto-Oncogenes* RNA, Messenger / genetics Receptor Protein-Tyrosine Kinases* Restriction Mapping Transcription, Genetic Tumor Cells, Cultured / enzymology
IF 7.971
Times Cited 475
DNA material pSV2 proto-ret (RDB01163)