| RRC ID |
48815
|
| Author |
Tashiro K, Tada H, Heilker R, Shirozu M, Nakano T, Honjo T.
|
| Title |
Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins.
|
| Journal |
Science
|
| Abstract |
A method was developed to clone, without the use of specific functional assays, complementary DNAs (cDNAs) that carry specific amino-terminal signal sequences, such as those encoding intercellular signal-transducing molecules and receptors. The vector used in this system directed the cell surface expression of interleukin-2 receptor fusion proteins when inserts with signal sequences were cloned in-frame with the correct orientation. An expression cDNA library was constructed from a bone marrow stromal cell line, which contained 5' portion-enriched cDNAs (the average size was 400 base pairs). Two cDNAs that encoded putative cytokine molecules, stromal cell-derived factor-1 alpha (SDF-1 alpha) and SDF-1 beta, which belong to the intercrine-macrophage inflammatory protein superfamily, were cloned.
|
| Volume |
261(5121)
|
| Pages |
600-3
|
| Published |
1993-7-30
|
| DOI |
10.1126/science.8342023
|
| PMID |
8342023
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Cell Line
Chemokine CXCL12
Chemokines, CXC*
Cloning, Molecular / methods*
Cytokines / chemistry
Cytokines / genetics*
Genetic Vectors
Molecular Sequence Data
Open Reading Frames
Protein Sorting Signals / chemistry
Protein Sorting Signals / genetics*
Protein Sorting Signals / metabolism
Receptors, Interleukin-2 / genetics
Recombinant Fusion Proteins / biosynthesis
Transfection
|
| IF |
41.846
|
| Times Cited |
611
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
mouse SDF-1 alpha cDNA (RDB06778)
mouse SDF-1 beta cDNA (RDB06779). |
