RRC ID 48820
Author Matsunami N, Hamaguchi Y, Yamamoto Y, Kuze K, Kangawa K, Matsuo H, Kawaichi M, Honjo T.
Title A protein binding to the J kappa recombination sequence of immunoglobulin genes contains a sequence related to the integrase motif.
Journal Nature
Abstract Site-specific recombination requires conserved DNA sequences specific to each system, and system-specific proteins that recognize specific DNA sequences. The site-specific recombinases seem to fall into at least two families, based on their protein structure and chemistry of strand breakage. One of these is the resolvase-invertase family, members of which seem to form a serine-phosphate linkage with DNA. Members of the other family, called the integrase family, contain a conserved tyrosine residue that forms a covalent linkage with the 3'-phosphate of DNA at the site of recombination. Structural comparison of integrases shows that these proteins share a highly conserved 40-residue motif. V-(D)-J recombination of the immunoglobulin gene requires conserved recombination signal sequences (RS) of a heptamer CACTGTG and a T-rich nonamer GGTTTTTGT, which are separated by a spacer sequence of either 12 or 23 bases We have recently purified, almost to homogeneity, a protein that specifically binds to the immunoglobulin J kappa RS containing the 23-base-pair spacer sequence. By synthesizing probes on the basis of partial amino-acid sequences of the purified protein, we have now isolated and characterized the complementary DNA of this protein. The amino-acid sequence deduced from the cDNA sequence reveals that the J kappa RS-binding protein has a sequence similar to the 40-residue motif of integrases of phages, bacteria and yeast, indicating that this protein could be involved in V-(D)-J recombination as a recombinase.
Volume 342(6252)
Pages 934-7
Published 1989-12-21
DOI 10.1038/342934a0
PMID 2556644
MeSH Amino Acid Sequence Animals B-Lymphocytes / immunology Base Sequence Cell Line DNA Nucleotidyltransferases / genetics* DNA Transposable Elements DNA-Binding Proteins / genetics DNA-Binding Proteins / isolation & purification Gene Rearrangement Genes, Immunoglobulin* Immunoglobulin kappa-Chains / genetics* Integrases Mice Molecular Sequence Data Plasmids Recombination, Genetic* Restriction Mapping Sequence Homology, Nucleic Acid
IF 42.779
Times Cited 149
DNA material RBP-J (RDB01801)