RRC ID 48822
Author Muramatsu M, Sankaranand VS, Anant S, Sugai M, Kinoshita K, Davidson NO, Honjo T.
Title Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells.
Journal J Biol Chem
Abstract We have identified a novel gene referred to as activation-induced deaminase (AID) by subtraction of cDNAs derived from switch-induced and uninduced murine B lymphoma CH12F3-2 cells, more than 80% of which switch exclusively to IgA upon stimulation. The amino acid sequence encoded by AID cDNA is homologous to that of apolipoprotein B (apoB) mRNA-editing enzyme, catalytic polypeptide 1 (APOBEC-1), a type of cytidine deaminase that constitutes a catalytic subunit for the apoB mRNA-editing complex. In vitro experiments using a glutathione S-transferase AID fusion protein revealed significant cytidine deaminase activity that is blocked by tetrahydrouridine and by zinc chelation. However, AID alone did neither demonstrate activity in C to U editing of apoB mRNA nor bind to AU-rich RNA targets. AID mRNA expression is induced in splenic B cells that were activated in vitro or by immunizations with sheep red blood cells. In situ hybridization of immunized spleen sections revealed the restricted expression of AID mRNA in developing germinal centers in which modulation of immunoglobulin gene information through somatic hypermutation and class switch recombination takes place. Taken together, these findings suggest that AID is a new member of the RNA-editing deaminase family and may play a role in genetic events in the germinal center B cell.
Volume 274(26)
Pages 18470-6
Published 1999-6-25
DOI 10.1074/jbc.274.26.18470
PII S0021-9258(19)87181-8
PMID 10373455
MeSH APOBEC-1 Deaminase Amino Acid Sequence Animals Apolipoproteins B B-Lymphocytes / enzymology* Cycloheximide / pharmacology Cytidine Deaminase / biosynthesis* Cytidine Deaminase / chemistry Cytidine Deaminase / genetics DNA, Complementary / isolation & purification Enzyme Induction / drug effects Gene Library Germinal Center / cytology* Germinal Center / enzymology Mice Molecular Sequence Data Open Reading Frames Phylogeny Protein Synthesis Inhibitors / pharmacology RNA Editing* RNA, Messenger / metabolism Sequence Alignment Tumor Cells, Cultured
IF 4.238
Times Cited 812
DNA material pmAID BSSK (RDB02864) pGST-AID (RDB03001).