RRC ID |
48836
|
Author |
Kitamura D, Kaneko H, Miyagoe Y, Ariyasu T, Watanabe T.
|
Title |
Isolation and characterization of a novel human gene expressed specifically in the cells of hematopoietic lineage.
|
Journal |
Nucleic Acids Res
|
Abstract |
A novel cDNA clone designated as HS1, which show an expression pattern limited to human hematopoietic cells, was isolated. About 2kb mRNA of the clone was accumulated in all the mature and immature lymphoid and myeloid cell lines tested, and two of three erythroblastoid cell lines, but not in any cell lines of non-hematopoietic tissues. The same mRNA was also detected in normal lymphoid and myeloid tissues and peripheral blood lymphocytes, granulocytes and macrophages, but again not in non-hematopoietic tissues. Nucleotide sequence of the HS1 predicts a protein of 486 amino acids (Mr 53,931). N-terminal half of the protein retains unique repeating motifs, each of which shows a significant homology with the helix-turn-helix DNA-binding motif of several proteins reported previously. C-terminal half of the protein retains a region conserved between non-receptor tyrosine kinases (src family), phospholipase C(PLC)-148 and the crk oncogene product. A unique feature of HS1 suggests that the protein may be involved in signal transduction and regulation of gene expression.
|
Volume |
17(22)
|
Pages |
9367-79
|
Published |
1989-11-25
|
PMID |
2587259
|
PMC |
PMC335138
|
MeSH |
Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Animals
Base Sequence
Blood Proteins / genetics*
Cell Line
Cloning, Molecular
Gene Expression*
Genes*
Humans
Leukocytes / metabolism*
Mice
Molecular Sequence Data
RNA, Messenger / genetics
Repetitive Sequences, Nucleic Acid
Restriction Mapping
Sequence Homology, Nucleic Acid
Tumor Cells, Cultured / metabolism
|
IF |
11.502
|
Times Cited |
144
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
Resource |
DNA material |
Human HS1 cDNA (RDB01286) |