RRC ID 48836
Author Kitamura D, Kaneko H, Miyagoe Y, Ariyasu T, Watanabe T.
Title Isolation and characterization of a novel human gene expressed specifically in the cells of hematopoietic lineage.
Journal Nucleic Acids Res
Abstract A novel cDNA clone designated as HS1, which show an expression pattern limited to human hematopoietic cells, was isolated. About 2kb mRNA of the clone was accumulated in all the mature and immature lymphoid and myeloid cell lines tested, and two of three erythroblastoid cell lines, but not in any cell lines of non-hematopoietic tissues. The same mRNA was also detected in normal lymphoid and myeloid tissues and peripheral blood lymphocytes, granulocytes and macrophages, but again not in non-hematopoietic tissues. Nucleotide sequence of the HS1 predicts a protein of 486 amino acids (Mr 53,931). N-terminal half of the protein retains unique repeating motifs, each of which shows a significant homology with the helix-turn-helix DNA-binding motif of several proteins reported previously. C-terminal half of the protein retains a region conserved between non-receptor tyrosine kinases (src family), phospholipase C(PLC)-148 and the crk oncogene product. A unique feature of HS1 suggests that the protein may be involved in signal transduction and regulation of gene expression.
Volume 17(22)
Pages 9367-79
Published 1989-11-25
PMID 2587259
PMC PMC335138
MeSH Amino Acid Sequence Animals Base Sequence Blood Proteins / genetics* Cell Line Cloning, Molecular Gene Expression* Genes* Humans Leukocytes / metabolism* Mice Molecular Sequence Data RNA, Messenger / genetics Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Nucleic Acid Tumor Cells, Cultured / metabolism
IF 11.147
Times Cited 144
DNA material Human HS1 cDNA (RDB01286)