Reference - Detail
|Author||Olesnicky EC, Bono JM, Bell L, Schachtner LT, Lybecker MC.|
|Title||The RNA-binding protein caper is required for sensory neuron development in Drosophila melanogaster.|
BACKGROUND:Alternative splicing mediated by RNA-binding proteins (RBPs) is emerging as a fundamental mechanism for the regulation of gene expression. Alternative splicing has been shown to be a widespread phenomenon that facilitates the diversification of gene products in a tissue-specific manner. Although defects in alternative splicing are rooted in many neurological disorders, only a small fraction of splicing factors have been investigated in detail.
RESULTS:We find that the splicing factor Caper is required for the development of multiple different mechanosensory neuron subtypes at multiple life stages in Drosophila melanogaster. Disruption of Caper function causes defects in dendrite morphogenesis of larval dendrite arborization neurons and neuronal positioning of embryonic proprioceptors, as well as the development and maintenance of adult mechanosensory bristles. Additionally, we find that Caper dysfunction results in aberrant locomotor behavior in adult flies. Transcriptome-wide analyses further support a role for Caper in alternative isoform regulation of genes that function in neurogenesis.
CONCLUSIONS:Our results provide the first evidence for a fundamental and broad requirement for the highly conserved splicing factor Caper in the development and maintenance of the nervous system and provide a framework for future studies on the detailed mechanism of Caper-mediated RNA regulation. Developmental Dynamics 246:610-624, 2017. © 2017 Wiley Periodicals, Inc.
|MeSH||Animals Chick Embryo Drosophila melanogaster / cytology* Drosophila melanogaster / metabolism* Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology Hemodynamics / genetics Hemodynamics / physiology Metamorphosis, Biological / genetics Metamorphosis, Biological / physiology Peripheral Vascular Diseases / genetics Peripheral Vascular Diseases / metabolism* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Sensory Receptor Cells / cytology* Sensory Receptor Cells / metabolism* Skin / cytology Skin / metabolism|