| RRC ID |
48866
|
| Author |
Tan SWS, Lee QY, Wong BSE, Cai Y, Baeg GH.
|
| Title |
Redox Homeostasis Plays Important Roles in the Maintenance of the Drosophila Testis Germline Stem Cells.
|
| Journal |
Stem Cell Reports
|
| Abstract |
Oxidative stress influences stem cell behavior by promoting the differentiation, proliferation, or apoptosis of stem cells. Thus, characterizing the effects of reactive oxygen species (ROS) on stem cell behavior provides insights into the significance of redox homeostasis in stem cell-associated diseases and efficient stem cell expansion for cellular therapies. We utilized the Drosophila testis as an in vivo model to examine the effects of ROS on germline stem cell (GSC) maintenance. High levels of ROS induced by alteration in Keap1/Nrf2 activity decreased GSC number by promoting precocious GSC differentiation. Notably, high ROS enhanced the transcription of the EGFR ligand spitz and the expression of phospho-Erk1/2, suggesting that high ROS-mediated GSC differentiation is through EGFR signaling. By contrast, testes with low ROS caused by Keap1 inhibition or antioxidant treatment showed an overgrowth of GSC-like cells. These findings suggest that redox homeostasis regulated by Keap1/Nrf2 signaling plays important roles in GSC maintenance.
|
| Volume |
9(1)
|
| Pages |
342-354
|
| Published |
2017-7-11
|
| DOI |
10.1016/j.stemcr.2017.05.034
|
| PII |
S2213-6711(17)30241-2
|
| PMID |
28669604
|
| PMC |
PMC5511110
|
| MeSH |
Animals
Cell Differentiation
Cell Proliferation
Drosophila
Drosophila Proteins / metabolism
Germ Cells / cytology*
Germ Cells / metabolism
Homeostasis
Kelch-Like ECH-Associated Protein 1 / metabolism
Male
NF-E2-Related Factor 2 / metabolism
Oxidation-Reduction
Oxidative Stress*
Reactive Oxygen Species / metabolism
Signal Transduction
Testis / cytology*
Testis / metabolism
|
| IF |
6.032
|
| Times Cited |
13
|
|
WOS Category
|
CELL & TISSUE ENGINEERING
CELL BIOLOGY
|
| Resource |
| Drosophila |
2286R-2
2286R-3
6343R-1
6343R-2 |
