Reference - Detail
| RRC ID | 48880 |
|---|---|
| Author | Fukuda M, Mikoshiba K. |
| Title | Synaptotagmin-like protein 1-3: a novel family of C-terminal-type tandem C2 proteins. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Synaptotagmins (Syt), rabphilin-3A, and Doc2 belong to a family of carboxyl terminal type (C-type) tandem C2 proteins and are thought to be involved in vesicular trafficking. We have cloned and characterized a novel family of C-type tandem C2 proteins, designated Slp1-3 (synaptotagmin-like protein 1-3). The Slp1-3 C2 domains show high homology to granuphilin-a C2 domains, but the amino-terminal domain of Slp1-3 does not contain any known protein motifs or a transmembrane domain. A subcellular fractionation study indicated that Slp1-3 proteins are peripheral membrane proteins. Phospholipid binding experiments indicated that Slp3 is a Ca(2+)-dependent isoform, but Slp1 and Slp2 are Ca(2+)-independent isoforms, because only the Slp3 C2A domain showed Ca(2+)-dependent phospholipid binding activity. The C-terminus of Slp1-3 also bound neurexin Ialpha in vitro, in the same manner as Syt family proteins, which may be important for the membrane association of Slp1-3. In addition, Slp family proteins are differentially distributed in different mouse tissues and at different developmental stages. |
| Volume | 281(5) |
| Pages | 1226-33 |
| Published | 2001-3-1 |
| DOI | 10.1006/bbrc.2001.4512 |
| PII | S0006-291X(01)94512-7 |
| PMID | 11243866 |
| MeSH | Amino Acid Sequence Animals COS Cells Calcium-Binding Proteins* Cloning, Molecular Dimerization Glycoproteins Intracellular Membranes / metabolism Membrane Glycoproteins / genetics* Membrane Glycoproteins / metabolism Membrane Proteins / chemistry Membrane Proteins / genetics* Membrane Proteins / metabolism Mice Molecular Sequence Data Nerve Tissue Proteins / genetics* Nerve Tissue Proteins / metabolism Neuropeptides Phospholipids / metabolism Phylogeny Protein Structure, Tertiary RNA, Messenger / biosynthesis Sequence Homology, Amino Acid Synaptotagmins Transfection Vesicular Transport Proteins |
| IF | 2.985 |
| Times Cited | 74 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 9 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| DNA material | pmRFP-C1-mouse Slp1 (RDB15089) |