論文 - 詳細
| RRC ID | 48882 |
|---|---|
| 著者 | Wang X, Olson JR, Rasoloson D, Ellenbecker M, Bailey J, Voronina E. |
| タイトル | Dynein light chain DLC-1 promotes localization and function of the PUF protein FBF-2 in germline progenitor cells. |
| ジャーナル | Development |
| Abstract |
PUF family translational repressors are conserved developmental regulators, but the molecular function provided by the regions flanking the PUF RNA-binding domain is unknown. In C. elegans, the PUF proteins FBF-1 and FBF-2 support germline progenitor maintenance by repressing production of meiotic proteins and use distinct mechanisms to repress their target mRNAs. We identify dynein light chain DLC-1 as an important regulator of FBF-2 function. DLC-1 directly binds to FBF-2 outside of the RNA-binding domain and promotes FBF-2 localization and function. By contrast, DLC-1 does not interact with FBF-1 and does not contribute to FBF-1 activity. Surprisingly, we find that the contribution of DLC-1 to FBF-2 activity is independent of the dynein motor. Our findings suggest that PUF protein localization and activity are mediated by sequences flanking the RNA-binding domain that bind specific molecular partners. Furthermore, these results identify a new role for DLC-1 in post-transcriptional regulation of gene expression. |
| 巻・号 | 143(24) |
| ページ | 4643-4653 |
| 公開日 | 2016-12-15 |
| DOI | 10.1242/dev.140921 |
| PII | dev.140921 |
| PMID | 27864381 |
| PMC | PMC5201030 |
| MeSH | Animals Binding Sites / genetics Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Dyneins / genetics Dyneins / metabolism* Gene Expression Regulation, Developmental / genetics* Protein Binding RNA Interference RNA, Small Interfering / genetics RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Stem Cells / cytology |
| IF | 5.611 |
| 引用数 | 11 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3153 |