| RRC ID |
48882
|
| 著者 |
Wang X, Olson JR, Rasoloson D, Ellenbecker M, Bailey J, Voronina E.
|
| タイトル |
Dynein light chain DLC-1 promotes localization and function of the PUF protein FBF-2 in germline progenitor cells.
|
| ジャーナル |
Development
|
| Abstract |
PUF family translational repressors are conserved developmental regulators, but the molecular function provided by the regions flanking the PUF RNA-binding domain is unknown. In C. elegans, the PUF proteins FBF-1 and FBF-2 support germline progenitor maintenance by repressing production of meiotic proteins and use distinct mechanisms to repress their target mRNAs. We identify dynein light chain DLC-1 as an important regulator of FBF-2 function. DLC-1 directly binds to FBF-2 outside of the RNA-binding domain and promotes FBF-2 localization and function. By contrast, DLC-1 does not interact with FBF-1 and does not contribute to FBF-1 activity. Surprisingly, we find that the contribution of DLC-1 to FBF-2 activity is independent of the dynein motor. Our findings suggest that PUF protein localization and activity are mediated by sequences flanking the RNA-binding domain that bind specific molecular partners. Furthermore, these results identify a new role for DLC-1 in post-transcriptional regulation of gene expression.
|
| 巻・号 |
143(24)
|
| ページ |
4643-4653
|
| 公開日 |
2016-12-15
|
| DOI |
10.1242/dev.140921
|
| PII |
dev.140921
|
| PMID |
27864381
|
| PMC |
PMC5201030
|
| MeSH |
Animals
Binding Sites / genetics
Caenorhabditis elegans / embryology*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Dyneins / genetics
Dyneins / metabolism*
Gene Expression Regulation, Developmental / genetics*
Protein Binding
RNA Interference
RNA, Small Interfering / genetics
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism*
Stem Cells / cytology
|
| IF |
5.611
|
| 引用数 |
11
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm3153 |
