| RRC ID |
48902
|
| Author |
Miki T, Awa M, Nishikawa Y, Kiyonaka S, Wakabayashi M, Ishihama Y, Hamachi I.
|
| Title |
A conditional proteomics approach to identify proteins involved in zinc homeostasis.
|
| Journal |
Nat Methods
|
| Abstract |
Zinc signaling and dynamics play significant roles in many physiological responses and diseases. To understand the physiological roles of zinc in detail, comprehensive identification of proteins under high concentration of mobile zinc ion is crucial. We developed a 'conditional proteomics' approach to identify proteins involved in zinc homeostasis based on a chemical proteomic strategy that utilizes designer zinc-responsive labeling reagents to tag such proteins and quantitative mass spectrometry for their identification. We used this method to elucidate zinc dyshomeostasis induced by nitric-oxide-triggered oxidative stress in glioma cells, and we unveiled dynamic changes of the zinc-related proteomes. Moreover, we characterized unknown zinc-rich vesicles generated by oxidative stress as endoplasmic-reticulum- and Golgi-related vesicles.
|
| Volume |
13(11)
|
| Pages |
931-937
|
| Published |
2016-11-1
|
| DOI |
10.1038/nmeth.3998
|
| PII |
nmeth.3998
|
| PMID |
27617391
|
| MeSH |
Binding Sites
Blotting, Western
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Cell Line, Tumor
Electrophoresis, Polyacrylamide Gel
Glioma
Green Fluorescent Proteins / genetics
HeLa Cells
Homeostasis / physiology*
Humans
Microscopy, Confocal
Nitric Oxide / pharmacology
Oxidative Stress / drug effects
Oxidative Stress / genetics
Protein Binding
Proteome / genetics
Proteome / metabolism*
Proteomics / methods*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Staining and Labeling
Zinc / metabolism*
|
| IF |
30.822
|
| Times Cited |
14
|
|
WOS Category
|
BIOCHEMICAL RESEARCH METHODS
|
| Resource |
| DNA material |
CALR-mCherry-KDEL (RDB15152)
PDIA3-mCherry-KDEL (RDB15153)
ENPL-mCherry-KDEL (RDB15154)
PPIA-mCherry (RDB15155)
ARF4-mCherry (RDB15156)
mCherry-EF1A1 (RDB15157)
mCherry-RAB1A (RDB15158)
mCherry-LC3B (RDB15159). |
