論文 - 詳細
| RRC ID | 48902 |
|---|---|
| 著者 | Miki T, Awa M, Nishikawa Y, Kiyonaka S, Wakabayashi M, Ishihama Y, Hamachi I. |
| タイトル | A conditional proteomics approach to identify proteins involved in zinc homeostasis. |
| ジャーナル | Nat Methods |
| Abstract |
Zinc signaling and dynamics play significant roles in many physiological responses and diseases. To understand the physiological roles of zinc in detail, comprehensive identification of proteins under high concentration of mobile zinc ion is crucial. We developed a 'conditional proteomics' approach to identify proteins involved in zinc homeostasis based on a chemical proteomic strategy that utilizes designer zinc-responsive labeling reagents to tag such proteins and quantitative mass spectrometry for their identification. We used this method to elucidate zinc dyshomeostasis induced by nitric-oxide-triggered oxidative stress in glioma cells, and we unveiled dynamic changes of the zinc-related proteomes. Moreover, we characterized unknown zinc-rich vesicles generated by oxidative stress as endoplasmic-reticulum- and Golgi-related vesicles. |
| 巻・号 | 13(11) |
| ページ | 931-937 |
| 公開日 | 2016-11-1 |
| DOI | 10.1038/nmeth.3998 |
| PII | nmeth.3998 |
| PMID | 27617391 |
| MeSH | Binding Sites Blotting, Western Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Line, Tumor Electrophoresis, Polyacrylamide Gel Glioma Green Fluorescent Proteins / genetics HeLa Cells Homeostasis / physiology* Humans Microscopy, Confocal Nitric Oxide / pharmacology Oxidative Stress / drug effects Oxidative Stress / genetics Protein Binding Proteome / genetics Proteome / metabolism* Proteomics / methods* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Staining and Labeling Zinc / metabolism* |
| IF | 30.822 |
| 引用数 | 14 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CALR-mCherry-KDEL (RDB15152) PDIA3-mCherry-KDEL (RDB15153) ENPL-mCherry-KDEL (RDB15154) PPIA-mCherry (RDB15155) ARF4-mCherry (RDB15156) mCherry-EF1A1 (RDB15157) mCherry-RAB1A (RDB15158) mCherry-LC3B (RDB15159). |