RRC ID 49196
Author Ohtaka K, Hori K, Kanno Y, Seo M, Ohta H.
Title Primitive Auxin Response without TIR1 and Aux/IAA in the Charophyte Alga Klebsormidium nitens.
Journal Plant Physiol.
Abstract The phytohormone auxin regulates many aspects of growth and development in land plants, but the origin and evolution of auxin signaling and response mechanisms remain largely unknown. Indeed, it remains to be investigated whether auxin-related pathways diverged before the emergence of land plants. To address this knowledge deficit, we analyzed auxin responses in the charophyte alga Klebsormidium nitens NIES-2285, whose ancestor diverged from a green algal ancestor during the evolution of land plants. This strain is the same as Klebsormidium flaccidum NIES-2285, for which the draft genome was sequenced in 2014, and was taxonomically reclassified as K. nitens This genome sequence revealed genes involved in auxin responses. Furthermore, the auxin indole-3-acetic acid (IAA) was detected in cultures of K. nitens, but K. nitens lacks the central regulators of the canonical auxin-signaling pathway found in land plants. Exogenous IAA inhibited cell division and cell elongation in K. nitens Inhibitors of auxin biosynthesis and of polar auxin transport also inhibited cell division and elongation. Moreover, exogenous IAA rapidly induced expression of a LATERAL ORGAN BOUNDARIES-DOMAIN transcription factor. These results suggest that K. nitens has acquired the part of the auxin system that regulates transcription and cell growth without the requirement for the central players that govern auxin signaling in land plants.
Volume 174(3)
Pages 1621-1632
Published 2017-7
DOI 10.1104/pp.17.00274
PII pp.17.00274
PMID 28533212
PMC PMC5490900
MeSH Biological Transport / drug effects Boronic Acids / pharmacology Cell Division / drug effects Charophyceae / drug effects Charophyceae / genetics Charophyceae / growth & development Charophyceae / metabolism* Gene Expression Profiling Gene Expression Regulation, Plant / drug effects Indoleacetic Acids / pharmacology* Microscopy, Fluorescence Plant Proteins / genetics Plant Proteins / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism Triiodobenzoic Acids / pharmacology
IF 6.305
Times Cited 0
WOS Category PLANT SCIENCES
Resource
Algae NIES-2285