RRC ID 49211
Author Ohbayashi N, Maruta Y, Ishida M, Fukuda M.
Title Melanoregulin regulates retrograde melanosome transport through interaction with the RILP-p150Glued complex in melanocytes.
Journal J Cell Sci
Abstract Melanoregulin (Mreg), a product of the dilute suppressor gene, has been implicated in the regulation of melanosome transport in mammalian epidermal melanocytes, given that Mreg deficiency was found to restore peripheral melanosome distribution from perinuclear melanosome aggregation in Rab27A-deficient melanocytes. However, the function of Mreg in melanosome transport has remained unclear. Here, we show that Mreg regulates microtubule-dependent retrograde melanosome transport through the dynein-dynactin motor complex. Mreg interacted with the C-terminal domain of Rab-interacting lysosomal protein (RILP) and formed a complex with RILP and p150(Glued) (also known as dynactin subunit 1, DCTN1), a component of the dynein-dynactin motor complex, in cultured cells. Overexpression of Mreg, RILP or both, in normal melanocytes induced perinuclear melanosome aggregation, whereas knockdown of Mreg or functional disruption of the dynein-dynactin motor complex restored peripheral melanosome distribution in Rab27A-deficient melanocytes. These findings reveal a new mechanism by which the dynein-dynactin motor complex recognizes Mreg on mature melanosomes through interaction with RILP and is involved in the centripetal movement of melanosomes.
Volume 125(Pt 6)
Pages 1508-18
Published 2012-3-15
DOI 10.1242/jcs.094185
PII jcs.094185
PMID 22275436
MeSH Adaptor Proteins, Signal Transducing Adaptor Proteins, Vesicular Transport Animals Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Line, Transformed Dynactin Complex Intracellular Signaling Peptides and Proteins Melanocytes / cytology Melanocytes / metabolism Melanocytes / physiology* Melanosomes / physiology* Mice Mice, Inbred Strains Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Multiprotein Complexes / genetics Multiprotein Complexes / metabolism Protein Transport / physiology
IF 4.573
Times Cited 35
DNA material pEGFP-C1-Mreg (RDB15182)