論文 - 詳細
| RRC ID | 49211 |
|---|---|
| 著者 | Ohbayashi N, Maruta Y, Ishida M, Fukuda M. |
| タイトル | Melanoregulin regulates retrograde melanosome transport through interaction with the RILP-p150Glued complex in melanocytes. |
| ジャーナル | J Cell Sci |
| Abstract |
Melanoregulin (Mreg), a product of the dilute suppressor gene, has been implicated in the regulation of melanosome transport in mammalian epidermal melanocytes, given that Mreg deficiency was found to restore peripheral melanosome distribution from perinuclear melanosome aggregation in Rab27A-deficient melanocytes. However, the function of Mreg in melanosome transport has remained unclear. Here, we show that Mreg regulates microtubule-dependent retrograde melanosome transport through the dynein-dynactin motor complex. Mreg interacted with the C-terminal domain of Rab-interacting lysosomal protein (RILP) and formed a complex with RILP and p150(Glued) (also known as dynactin subunit 1, DCTN1), a component of the dynein-dynactin motor complex, in cultured cells. Overexpression of Mreg, RILP or both, in normal melanocytes induced perinuclear melanosome aggregation, whereas knockdown of Mreg or functional disruption of the dynein-dynactin motor complex restored peripheral melanosome distribution in Rab27A-deficient melanocytes. These findings reveal a new mechanism by which the dynein-dynactin motor complex recognizes Mreg on mature melanosomes through interaction with RILP and is involved in the centripetal movement of melanosomes. |
| 巻・号 | 125(Pt 6) |
| ページ | 1508-18 |
| 公開日 | 2012-3-15 |
| DOI | 10.1242/jcs.094185 |
| PII | jcs.094185 |
| PMID | 22275436 |
| MeSH | Adaptor Proteins, Signal Transducing Adaptor Proteins, Vesicular Transport Animals Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Line, Transformed Dynactin Complex Intracellular Signaling Peptides and Proteins Melanocytes / cytology Melanocytes / metabolism Melanocytes / physiology* Melanosomes / physiology* Mice Mice, Inbred Strains Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Multiprotein Complexes / genetics Multiprotein Complexes / metabolism Protein Transport / physiology |
| IF | 4.573 |
| 引用数 | 35 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pEGFP-C1-Mreg (RDB15182) |