論文 - 詳細
| RRC ID | 49243 |
|---|---|
| 著者 | Qin C, Xiao C, Su Y, Zheng H, Xu T, Lu J, Luo P, Zhang J. |
| タイトル | Tisp40 deficiency attenuates renal ischemia reperfusion injury induced apoptosis of tubular epithelial cells. |
| ジャーナル | Exp Cell Res |
| Abstract |
Renal ischemia reperfusion (IR) is a major cause of acute kidney injury (AKI) and no effective treatments have been established. Tisp40 is a transcription factor of the CREB/ATF family and involves in cell apoptosis, proliferation and differentiation, but its role in renal IR remains unknown. Here, we investigated the role of Tisp40 in renal IR injury. In vivo, Tisp40 knockout (KO) and wild-type (WT) mice were subjected to thirty minutes of bilateral renal ischemia and 48h reperfusion, the blood and kidneys were harvested for analysis. In vitro, Tisp40 overexpression and vector cells were subjected to hypoxia/reoxygenation (HR), the apoptosis rate and the expressions of related proteins were measured. Following IR, the expressions of Tisp40 protein, serum creatinine (sCr), blood urea nitrogen (BUN) and apoptosis of tubular cells were significantly increased in WT mice. However, Tisp40 deficiency significantly attenuated the increase of sCr, BUN and apoptosis of tubular cells. Following HR, apoptosis of tubular cells was increased in Tisp40 overexpression cells compared with vector cells. Mechanistically, Tisp40 promoted the expressions of C/EBP homologous protein (CHOP), Bax and Cleaved caspase3 and suppressed the expression of Bcl-2 in renal IR injury. In conclusion, Tisp40 aggravates tubular cells apoptosis in renal IR injury. |
| 巻・号 | 359(1) |
| ページ | 138-144 |
| 公開日 | 2017-10-1 |
| DOI | 10.1016/j.yexcr.2017.07.038 |
| PII | S0014-4827(17)30409-3 |
| PMID | 28778797 |
| MeSH | Animals Apoptosis* Cyclic AMP Response Element-Binding Protein / deficiency* Cyclic AMP Response Element-Binding Protein / metabolism Epithelial Cells / metabolism* Epithelial Cells / pathology Hypoxia / complications Hypoxia / pathology Kidney Tubules / abnormalities Kidney Tubules / pathology* Kidney Tubules / physiopathology Mice, Inbred C57BL Mice, Knockout Oxygen Reperfusion Injury / metabolism* Reperfusion Injury / pathology* Reperfusion Injury / physiopathology Transcription Factor CHOP / metabolism |
| IF | 3.383 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01942 |