論文 - 詳細
| RRC ID | 49258 |
|---|---|
| 著者 | Niwa S, Tao L, Lu SY, Liew GM, Feng W, Nachury MV, Shen K. |
| タイトル | BORC Regulates the Axonal Transport of Synaptic Vesicle Precursors by Activating ARL-8. |
| ジャーナル | Curr Biol |
| Abstract |
Axonal transport of synaptic vesicle precursors (SVPs) is essential for synapse development and function. The conserved ARF-like small GTPase ARL-8 is localized to SVPs and directly activates UNC-104/KIF1A, the axonal-transport kinesin for SVPs in C. elegans. It is not clear how ARL-8 is activated in this process. Here we show that part of the BLOC-1-related complex (BORC), previously shown to regulate lysosomal transport, is required to recruit and activate ARL-8 on SVPs. We found mutations in six BORC subunits-blos-1/BLOS1, blos-2/BLOS2, snpn-1/Snapin, sam-4/Myrlysin, blos-7/Lyspersin, and blos-9/MEF2BNB-cause defects in axonal transport of SVPs, leading to ectopic accumulation of synaptic vesicles in the proximal axon. This phenotype is suppressed by constitutively active arl-8 or unc-104 mutants. Furthermore, SAM-4/Myrlysin, a subunit of BORC, promotes the GDP-to-GTP exchange of ARL-8 in vitro and recruits ARL-8 onto SVPs in vivo. Thus, BORC regulates the axonal transport of synaptic materials and synapse formation by controlling the nucleotide state of ARL-8. Interestingly, the other two subunits of BORC essential for lysosomal transport, kxd-1/KXD1 and blos-8/Diaskedin, are not required for the SVP transport, suggesting distinct subunit requirements for lysosomal and SVP trafficking. |
| 巻・号 | 27(17) |
| ページ | 2569-2578.e4 |
| 公開日 | 2017-9-11 |
| DOI | 10.1016/j.cub.2017.07.013 |
| PII | S0960-9822(17)30871-0 |
| PMID | 28823680 |
| PMC | PMC5693321 |
| MeSH | Animals Axonal Transport / genetics* Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Synaptic Vesicles / physiology* |
| IF | 9.601 |
| 引用数 | 20 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1159 tm1892 tm2388 tm3828 tm6384 |