論文 - 詳細
| RRC ID | 49284 |
|---|---|
| 著者 | Itoh A, Fukata Y, Miyanaka H, Nonaka Y, Ogawa T, Nakamura T, Nishi N. |
| タイトル | Optimization of the inter-domain structure of galectin-9 for recombinant production. |
| ジャーナル | Glycobiology |
| Abstract |
We previously developed a stable form of galectin-9, an immunomodulatory animal lectin with a truncated linker peptide (G9Null), to overcome the protease sensitivity of wild-type galectin-9. G9Null is highly resistant to proteolysis, while the modification marginally improved the low solubility of the wild-type protein. To increase its solubility, we further modified the remaining linker region of G9Null. A 10-amino acid deletion with a single amino acid substitution resulted in an ∼400% increase in solubility and yield without an adverse effect on its biological activity. This mutant protein might be useful for large-scale recombinant production needed for evaluation of the therapeutic potential of galectin-9. |
| 巻・号 | 23(8) |
| ページ | 920-5 |
| 公開日 | 2013-8-1 |
| DOI | 10.1093/glycob/cwt023 |
| PII | cwt023 |
| PMID | 23507964 |
| MeSH | Amino Acid Substitution* Animals Galectins / biosynthesis Galectins / chemistry Galectins / genetics* Protein Structure, Tertiary Recombinant Fusion Proteins / biosynthesis Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / genetics Solubility |
| IF | 4.06 |
| 引用数 | 6 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pET-ssG9 (RDB15282) |