RRC ID 49287
Author Gigant E, Stefanutti M, Laband K, Gluszek-Kustusz A, Edwards F, Lacroix B, Maton G, Canman JC, Welburn JPI, Dumont J.
Title Inhibition of ectopic microtubule assembly by the kinesin-13 KLP-7 prevents chromosome segregation and cytokinesis defects in oocytes.
Journal Development
Abstract In most species, oocytes lack centrosomes. Accurate meiotic spindle assembly and chromosome segregation - essential to prevent miscarriage or developmental defects - thus occur through atypical mechanisms that are not well characterized. Using quantitative in vitro and in vivo functional assays in the C. elegans oocyte, we provide novel evidence that the kinesin-13 KLP-7 promotes destabilization of the whole cellular microtubule network. By counteracting ectopic microtubule assembly and disorganization of the microtubule network, this function is strictly required for spindle organization, chromosome segregation and cytokinesis in meiotic cells. Strikingly, when centrosome activity was experimentally reduced, the absence of KLP-7 or the mammalian kinesin-13 protein MCAK (KIF2C) also resulted in ectopic microtubule asters during mitosis in C. elegans zygotes or HeLa cells, respectively. Our results highlight the general function of kinesin-13 microtubule depolymerases in preventing ectopic, spontaneous microtubule assembly when centrosome activity is defective or absent, which would otherwise lead to spindle microtubule disorganization and aneuploidy.
Volume 144(9)
Pages 1674-1686
Published 2017-5-1
DOI 10.1242/dev.147504
PII dev.147504
PMID 28289130
PMC PMC5450848
MeSH Caenorhabditis elegans Proteins / metabolism* Chromosome Segregation* Cytokinesis* HeLa Cells Humans Imaging, Three-Dimensional Kinesins / metabolism* Meiosis Microtubules / metabolism* Oocytes / cytology* Oocytes / metabolism* Spindle Apparatus / metabolism
IF 5.611
Times Cited 9
C.elegans tm2143