論文 - 詳細
| RRC ID | 49304 |
|---|---|
| 著者 | Luo L, Siah CK, Cai Y. |
| タイトル | Engrailed acts with Nejire to control decapentaplegic expression in the Drosophila ovarian stem cell niche. |
| ジャーナル | Development |
| Abstract |
Homeostasis of adult tissues is maintained by a small number of stem cells, which are sustained by their niches. In the Drosophila female germline stem cell (GSC) niche, Decapentaplegic (Dpp) is the primary factor that promotes GSC self-renewal. However, the mechanism regulating dpp expression in the niche is largely unknown. Here, we identify a 2.0 kb fragment located in a 5' cis-regulatory region of the dpp locus containing enhancer activity that drives its expression in the niche. This region is distinct from a previously characterized 3' cis-regulatory enhancer responsible for dpp expression in imaginal discs. Our data demonstrate that Engrailed, a homeodomain-containing transcription factor that serves as a cap cell marker, binds to this region and regulates dpp expression in cap cells. Further data suggest that En forms a complex with Nejire (Nej), the Drosophila ortholog of histone acetyltransferase CBP/p300, and directs Nej to this cis-regulatory region where Nej functions as the co-activator for dpp expression. Therefore, our study defines the molecular pathway controlling dpp expression in the Drosophila ovarian stem cell niche. |
| 巻・号 | 144(18) |
| ページ | 3224-3231 |
| 公開日 | 2017-9-15 |
| DOI | 10.1242/dev.145474 |
| PII | 144/18/3224 |
| PMID | 28928281 |
| MeSH | Animals Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / metabolism* Female Gene Expression Regulation Genes, Reporter Homeodomain Proteins / metabolism* Ovary / cytology* Ovary / metabolism* Protein Binding Regulatory Sequences, Nucleic Acid / genetics Stem Cell Niche* / genetics Stem Cells / cytology Stem Cells / metabolism Transcription Factors / metabolism* p300-CBP Transcription Factors / metabolism* |
| IF | 5.611 |
| 引用数 | 6 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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