RRC ID 49320
Author Tsutsui H, Jinno Y, Tomita A, Niino Y, Yamada Y, Mikoshiba K, Miyawaki A, Okamura Y.
Title Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase.
Journal J Physiol
Abstract   One of the most awaited techniques in modern physiology is the sensitive detection of spatiotemporal electrical activity in a complex network of excitable cells. The use of genetically encoded voltage probes has been expected to enable such analysis. However, in spite of recent progress, existing probes still suffer from low signal amplitude and/or kinetics too slow to detect fast electrical activity. Here, we have developed an improved voltage probe named Mermaid2, which is based on the voltage-sensor domain of the voltage-sensing phosphatase from Ciona intestinalis and Förster energy transfer between a pair of fluorescent proteins. In mammalian cells, Mermaid2 permits ratiometric readouts of fractional changes of more than 50% over a physiologically relevant voltage range with fast kinetics, and it was used to follow a train of action potentials at frequencies of up to 150 Hz. Mermaid2 was also able to detect single action potentials and subthreshold voltage responses in hippocampal neurons in vitro, in addition to cortical electrical activity evoked by sound stimuli in single trials in living mice.
Volume 591(18)
Pages 4427-37
Published 2013-9-15
DOI 10.1113/jphysiol.2013.257048
PII jphysiol.2013.257048
PMID 23836686
PMC PMC3784191
MeSH Action Potentials* Animals Fluorescence Resonance Energy Transfer Functional Neuroimaging / methods* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism HEK293 Cells Humans Mice Neurons / physiology Optogenetics / methods* Phosphoric Monoester Hydrolases / genetics* Phosphoric Monoester Hydrolases / metabolism Rats Xenopus
IF 4.54
Times Cited 39
DNA material Mermaid2 (RDB15263)