論文 - 詳細
| RRC ID | 49320 |
|---|---|
| 著者 | Tsutsui H, Jinno Y, Tomita A, Niino Y, Yamada Y, Mikoshiba K, Miyawaki A, Okamura Y. |
| タイトル | Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase. |
| ジャーナル | J Physiol |
| Abstract |
One of the most awaited techniques in modern physiology is the sensitive detection of spatiotemporal electrical activity in a complex network of excitable cells. The use of genetically encoded voltage probes has been expected to enable such analysis. However, in spite of recent progress, existing probes still suffer from low signal amplitude and/or kinetics too slow to detect fast electrical activity. Here, we have developed an improved voltage probe named Mermaid2, which is based on the voltage-sensor domain of the voltage-sensing phosphatase from Ciona intestinalis and Förster energy transfer between a pair of fluorescent proteins. In mammalian cells, Mermaid2 permits ratiometric readouts of fractional changes of more than 50% over a physiologically relevant voltage range with fast kinetics, and it was used to follow a train of action potentials at frequencies of up to 150 Hz. Mermaid2 was also able to detect single action potentials and subthreshold voltage responses in hippocampal neurons in vitro, in addition to cortical electrical activity evoked by sound stimuli in single trials in living mice. |
| 巻・号 | 591(18) |
| ページ | 4427-37 |
| 公開日 | 2013-9-15 |
| DOI | 10.1113/jphysiol.2013.257048 |
| PII | jphysiol.2013.257048 |
| PMID | 23836686 |
| PMC | PMC3784191 |
| MeSH | Action Potentials* Animals Fluorescence Resonance Energy Transfer Functional Neuroimaging / methods* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism HEK293 Cells Humans Mice Neurons / physiology Optogenetics / methods* Phosphoric Monoester Hydrolases / genetics* Phosphoric Monoester Hydrolases / metabolism Rats Xenopus |
| IF | 4.54 |
| 引用数 | 39 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Podcasts |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Mermaid2 (RDB15263) |