RRC ID 49325
Author Fairchild MJ, Islam F, Tanentzapf G.
Title Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.
Journal PLoS Genet
Abstract Spermatogenesis is a dynamic developmental process requiring precisely timed transitions between discrete stages. Specifically, the germline undergoes three transitions: from mitotic spermatogonia to spermatocytes, from meiotic spermatocytes to spermatids, and from morphogenetic spermatids to spermatozoa. The somatic cells of the testis provide essential support to the germline throughout spermatogenesis, but their precise role during these developmental transitions has not been comprehensively explored. Here, we describe the identification and characterization of genes that are required in the somatic cells of the Drosophila melanogaster testis for progress through spermatogenesis. Phenotypic analysis of candidate genes pinpointed the stage of germline development disrupted. Bioinformatic analysis revealed that particular gene classes were associated with specific developmental transitions. Requirement for genes associated with endocytosis, cell polarity, and microtubule-based transport corresponded with the development of spermatogonia, spermatocytes, and spermatids, respectively. Overall, we identify mechanisms that act specifically in the somatic cells of the testis to regulate spermatogenesis.
Volume 13(9)
Pages e1007026
Published 2017-9-1
DOI 10.1371/journal.pgen.1007026
PII PGENETICS-D-17-01178
PMID 28957323
PMC PMC5634645
MeSH Animals Cell Differentiation Computational Biology Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Female Gene Expression Regulation Gene Regulatory Networks* Male Meiosis / genetics Mitosis / genetics RNA Interference Spermatids / growth & development Spermatogenesis* Spermatozoa / growth & development Testis / cytology* Tubulin / genetics Tubulin / metabolism rab5 GTP-Binding Proteins / genetics rab5 GTP-Binding Proteins / metabolism
IF 5.175
Times Cited 10
Resource
Drosophila 1403R-1 4173R-1 4807R-1 9749R-3 4032R-2 10067R-2 7478R-2 5178R-1 4376R-1 8308R-3 7462R-1 9579R-3 9968R-1 3166R-3 11579R-1 6174R-2 7558R-1 7940R-2 11678R-1 10954R-1 4560R-2 6546R-1 5870R-3 3401R-1 4869R-4 1480R-1 5680R-1 1479R-2 18355R-2 9379R-3 18408R-3 4427R-2 12530R-3 2008R-2 7212R-2 11924R-2 10802R-1 1441R-2 14763R-1 14995R-3 15369R-2 17237R-3 1141R-1 4586R-2 7794R-1 8086R-2 8800R-1 3937R-2 3937R-4 9553R-3 15224R-1 3244R-3 4832R-1 9446R-1 3637R-2 6384R-1 10540R-1 5069R-2 14622R-1 9695R-2 6224R-1 8269R-3 10846R-2 10846R-1 5526R-2 15804R-1 7507R-2 3723R-3 1768R-1 1938R-4 3727R-1 9885R-1 1897R-1 1873R-4 5803R-3 10724R-2 17566R-1 1106R-1 8384R-4 10971R-1 16902R-1 9325R-1 4590R-2 11605R-1 2275R-1 11759R-2 13956R-2 7765R-1 5433R-2 1453R-3 5300R-1 10275R-1 31095R-3 1825R-1 5784R-2 14228R-2 1742R-3 17927R-4 1019R-3 10701R-1 4696R-4 5000R-3 3936R-2 5703R-1 14895R-1 10695R-1 9493R-3 5939R-2 9763R-2 6433R-4 2845R-3 9635R-3 4636R-2 3273R-2 3283R-1 18076R-3 6534R-3 2272R-1 1536R-4 1571R-2 10076R-2 12114R-1 4931R-1 7873R-2 7524R-1 17957R-1 6238R-4 7954R-1 7152R-1 2981R-2 11326R-2 2956R-1 10491R-1 1520R-2 1322R-1 1913R-2