Reference - Detail
| RRC ID | 49348 |
|---|---|
| Author | Sasaki T, Sato Y, Higashiyama T, Sasaki N. |
| Title | Live imaging reveals the dynamics and regulation of mitochondrial nucleoids during the cell cycle in Fucci2-HeLa cells. |
| Journal | Sci Rep |
| Abstract |
Mitochondrial DNA (mtDNA) is organized in nucleoprotein complexes called mitochondrial nucleoids (mt-nucleoids), which are critical units of mtDNA replication and transmission. In humans, several hundreds of mt-nucleoids exist in a cell. However, how numerous mt-nucleoids are maintained during the cell cycle remains elusive, because cell cycle synchronization procedures affect mtDNA replication. Here, we analyzed regulation of the maintenance of mt-nucleoids in the cell cycle, using a fluorescent cell cycle indicator, Fucci2. Live imaging of mt-nucleoids with higher temporal resolution showed frequent attachment and detachment of mt-nucleoids throughout the cell cycle. TFAM, an mtDNA packaging protein, was involved in the regulation of this dynamic process, which was important for maintaining proper mt-nucleoid number. Both an increase in mt-nucleoid number and activation of mtDNA replication occurred during S phase. To increase mt-nucleoid number, mtDNA replication, but not nuclear DNA replication, was necessary. We propose that these dynamic and regulatory processes in the cell cycle maintain several hundred mt-nucleoids in proliferating cells. |
| Volume | 7(1) |
| Pages | 11257 |
| Published | 2017-9-12 |
| DOI | 10.1038/s41598-017-10843-8 |
| PII | 10.1038/s41598-017-10843-8 |
| PMID | 28900194 |
| PMC | PMC5595809 |
| MeSH | Cell Cycle* DNA, Mitochondrial / metabolism* DNA-Binding Proteins / metabolism* Epithelial Cells / physiology* Fluorescent Dyes / analysis HeLa Cells Humans Intravital Microscopy* Microscopy, Fluorescence Mitochondrial Proteins / metabolism* Nucleoproteins / metabolism* Staining and Labeling Transcription Factors / metabolism* |
| IF | 3.998 |
| Times Cited | 10 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 21 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) HeLa/Fucci2(RCB2867) |