| RRC ID |
49456
|
| 著者 |
Lee SY, Kim BJ, Kim H, Won YS, Jeon CO, Jeong J, Lee SH, Lim JH, Lee SH, Kim CK, Kook YH, Kim BJ.
|
| タイトル |
Mycobacterium paraintracellulare sp. nov., for the genotype INT-1 of Mycobacterium intracellulare.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
Three mycobacterial strains, isolated from independent Korean patients with pulmonary infections, belonging to the Mycobacterium intracellulare genotype 1 (INT-1) were characterized using a polyphasic approach. The sequences of the 16S rRNA gene and internal transcribed spacer 1 (ITS1) of the INT-1 strains were identical to those of Mycobacterium intracellulare ATCC 13950T. However, multilocus sequence typing (MLST) analysis targeting five housekeeping genes (hsp65, rpoB, argG, gnd and pgm) revealed the phylogenetic separation of these strains from M. intracellulare ATCC 13950T. DNA-DNA hybridization values of >70 % confirmed that the three isolates belong to the same species, while the values of <70 % between one of them and the type strains of M. intracellulare and Mycobacterium chimaera confirmed their belonging to a distinct species. In addition, phenotypic characteristics such as positive growth on MacConkey agar and in acidic broth culture, unique matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS profiles of lipids, and unique mycolic acids profiles further supported the taxonomic status of these strains as representatives of a novel species of the Mycobacterium avium complex named Mycobacterium paraintracellulare. The type strain is MOTT64T (=KCTC 29084T=JCM 30622T).
|
| 巻・号 |
66(8)
|
| ページ |
3132-3141
|
| 公開日 |
2016-8-1
|
| DOI |
10.1099/ijsem.0.001158
|
| PMID |
27189351
|
| MeSH |
Aged
Aged, 80 and over
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
DNA, Ribosomal Spacer / genetics
Female
Genes, Bacterial
Humans
Male
Middle Aged
Multilocus Sequence Typing
Mycobacterium Infections / microbiology
Mycobacterium avium Complex / classification*
Mycobacterium avium Complex / genetics
Mycobacterium avium Complex / isolation & purification
Mycolic Acids / chemistry
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Republic of Korea
Sequence Analysis, DNA
Sputum / microbiology*
|
| IF |
2.415
|
| 引用数 |
15
|
| リソース情報 |
| 一般微生物 |
JCM 30622
JCM 14737 |
