論文 - 詳細
| RRC ID | 49530 |
|---|---|
| 著者 | Watanabe Y, Honda S, Konishi A, Arakawa S, Murohashi M, Yamaguchi H, Torii S, Tanabe M, Tanaka S, Warabi E, Shimizu S. |
| タイトル | Autophagy controls centrosome number by degrading Cep63. |
| ジャーナル | Nat Commun |
| Abstract |
Centrosome number is associated with the chromosome segregation and genomic stability. The ubiquitin-proteasome system is considered to be the main regulator of centrosome number. However, here we show that autophagy also regulates the number of centrosomes. Autophagy-deficient cells carry extra centrosomes. The autophagic regulation of centrosome number is dependent on a centrosomal protein of 63 (Cep63) given that cells lacking autophagy contain multiple Cep63 dots that are engulfed and digested by autophagy in wild-type cells, and that the upregulation of Cep63 increases centrosome number. Cep63 is recruited to autophagosomes via interaction with p62, a molecule crucial for selective autophagy. In vivo, hematopoietic cells from autophagy-deficient and p62-/- mice also contained multiple centrosomes. These results indicate that autophagy controls centrosome number by degrading Cep63. |
| 巻・号 | 7 |
| ページ | 13508 |
| 公開日 | 2016-11-21 |
| DOI | 10.1038/ncomms13508 |
| PII | ncomms13508 |
| PMID | 27869116 |
| PMC | PMC5473638 |
| MeSH | Animals Autophagy* Autophagy-Related Protein 5 / metabolism Autophagy-Related Protein 7 / genetics Autophagy-Related Protein 7 / metabolism Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cells, Cultured Centrosome* Humans Mice Mice, Knockout Neoplasm Proteins / genetics Neoplasm Proteins / metabolism* Sequestosome-1 Protein / genetics Sequestosome-1 Protein / metabolism |
| IF | 12.121 |
| 引用数 | 11 |
| リソース情報 | |
| 遺伝子材料 | pMSCV-GFP-mCentrin2 (RDB15401) pMSCV-GFP-hCep63 (RDB15403) pMSCV-3xFLAG-hCep63 (RDB15404) pMSCV-3xFLAG-mp62-del PB1 (RDB15405) pMSCV-3xFLAG-mp62-del Zinc (RDB15406) pMSCV-3xFLAG-mp62-PB1/Zinc (RDB15407) pMSCV-3xFLAG-mp62-del UBA (RDB15408). |