Reference - Detail
RRC ID | 49570 |
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Author | Saito K, Tatsuguchi K, Murakoshi I, Hirano H. |
Title | cDNA cloning and expression of cysteine synthase B localized in chloroplasts of Spinacia oleracea. |
Journal | FEBS Lett |
Abstract |
The cDNA clones for cysteine synthase B, which is localized in chloroplasts of Spinacia oleracea L., were isolated by screening a library with synthetic oligonucleotides encoding a partial peptide sequence of the purified protein. Nucleotide sequence analysis revealed an open reading frame encoding a polypeptide of 383 amino acids containing a putative transit peptide of 52 amino acids. A bacterial expression vector of the cDNA clone could genetically complement an Escherichia coli auxotroph lacking cysteine synthase and could produce the functionally active and immuno-reactive cysteine synthase in E. coli. RNA blot hybridization suggested that the transcripts were primarily accumulated in leaves of spinach. |
Volume | 324(3) |
Pages | 247-52 |
Published | 1993-6-21 |
DOI | 10.1016/0014-5793(93)80127-g |
PII | 0014-5793(93)80127-G |
PMID | 8405359 |
MeSH | Amino Acid Sequence Base Sequence Chloroplasts / enzymology* Cloning, Molecular Consensus Sequence DNA, Complementary / genetics Gene Expression Genes, Plant Genetic Complementation Test Lyases / genetics* Molecular Sequence Data Oligonucleotide Probes / chemistry Plant Proteins / genetics Plant Proteins / metabolism Sequence Alignment Sequence Homology, Amino Acid Vegetables / genetics |
IF | 3.057 |
Times Cited | 53 |
Resource | |
DNA material | PCSB II (RDB01537) |