RRC ID 4958
Author Engl C, Jovanovic G, Lloyd LJ, Murray H, Spitaler M, Ying L, Errington J, Buck M.
Title In vivo localizations of membrane stress controllers PspA and PspG in Escherichia coli.
Journal Mol Microbiol
Abstract The phage shock protein (Psp) response in Gram-negative bacteria counteracts membrane stress. Transcription of the PspF regulon (pspABCDE and pspG) in Escherichia coli is induced upon stresses that dissipate the proton motive force (pmf). Using GFP fusions we have visualized the subcellular localizations of PspA (a negative regulator and effector of Psp) and PspG (an effector of Psp). It has previously been proposed that PspA evenly coates the inner membrane of the cell. We now demonstrate that instead of uniformly covering the entire cell, PspA (and PspG) is highly organized into what appear to be distinct functional classes (complexes at the cell pole and the lateral cell wall). Real-time observations revealed lateral PspA and PspG complexes are highly mobile, but absent in cells lacking MreB. Without the MreB cytoskeleton, induction of the Psp response is still observed, yet these cells fail to maintain pmf under stress conditions. The two spatial subspecies therefore appear to be dynamically and functionally distinct with the polar clusters being associated with sensory function and the mobile complexes with maintenance of pmf.
Volume 73(3)
Pages 382-96
Published 2009-8-1
DOI 10.1111/j.1365-2958.2009.06776.x
PMID 19555453
PMC PMC2763126
MeSH Bacterial Proteins / genetics Bacterial Proteins / metabolism* Cytoskeleton / metabolism DNA, Bacterial / genetics Escherichia coli / genetics* Escherichia coli / metabolism Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Heat-Shock Proteins / genetics Heat-Shock Proteins / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Regulon
IF 3.649
Times Cited 48
Prokaryotes E. coli ME7986 (MG1655)