RRC ID 49611
著者 Kawada J, Kaneda S, Kirihara T, Maroof A, Levi T, Eggan K, Fujii T, Ikeuchi Y.
タイトル Generation of a Motor Nerve Organoid with Human Stem Cell-Derived Neurons.
ジャーナル Stem Cell Reports
Abstract During development, axons spontaneously assemble into a fascicle to form nerves and tracts in the nervous system as they extend within a spatially constrained path. However, understanding of the axonal fascicle has been hampered by lack of an in vitro model system. Here, we report generation of a nerve organoid composed of a robust fascicle of axons extended from a spheroid of human stem cell-derived motor neurons within our custom-designed microdevice. The device is equipped with a narrow channel providing a microenvironment that facilitates the growing axons to spontaneously assemble into a unidirectional fascicle. The fascicle was specifically made with axons. We found that it was electrically active and elastic and could serve as a model to evaluate degeneration of axons in vitro. This nerve organoid model should facilitate future studies on the development of the axonal fascicle and drug screening for diseases affecting axon fascicles.
巻・号 9(5)
ページ 1441-1449
公開日 2017-11-7
DOI 10.1016/j.stemcr.2017.09.021
PII S2213-6711(17)30428-9
PMID 29107592
PMC PMC5831012
ヒト・動物細胞 409B2(HPS0076) 610B1(HPS0331)