RRC ID 49648
Author Min S, Yoon W, Cho H, Chung J.
Title Misato underlies visceral myopathy in Drosophila.
Journal Sci Rep
Abstract Genetic mechanisms for the pathogenesis of visceral myopathy (VM) have been rarely demonstrated. Here we report the visceral role of misato (mst) in Drosophila and its implications for the pathogenesis of VM. Depletion of mst using three independent RNAi lines expressed by a pan-muscular driver elicited characteristic symptoms of VM, such as abnormal dilation of intestinal tracts, reduced gut motility, feeding defects, and decreased life span. By contrast, exaggerated expression of mst reduced intestine diameters, but increased intestinal motilities along with thickened muscle fibers, demonstrating a critical role of mst in the visceral muscle. Mst expression was detected in the adult intestine with its prominent localization to actin filaments and was required for maintenance of intestinal tubulin and actomyosin structures. Consistent with the subcellular localization of Mst, the intestinal defects induced by mst depletion were dramatically rescued by exogenous expression of an actin member. Upon ageing the intestinal defects were deteriorative with marked increase of apoptotic responses in the visceral muscle. Taken together, we propose the impairment of actomyosin structures induced by mst depletion in the visceral muscle as a pathogenic mechanism for VM.
Volume 7(1)
Pages 17700
Published 2017-12-18
DOI 10.1038/s41598-017-17961-3
PII 10.1038/s41598-017-17961-3
PMID 29255146
PMC PMC5735100
MeSH Actins / metabolism Actomyosin / metabolism Animals Apoptosis / physiology Cell Cycle Proteins / genetics* Cell Cycle Proteins / metabolism Cytoskeletal Proteins / genetics* Cytoskeletal Proteins / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / physiology Intestinal Pseudo-Obstruction / genetics* Intestinal Pseudo-Obstruction / physiopathology* Intestines / physiology Muscles / metabolism RNA Interference Tubulin / metabolism
IF 3.998
Times Cited 1
Drosophila 1424R-1