| RRC ID |
4964
|
| Author |
Inwood WB, Hall JA, Kim KS, Demirkhanyan L, Wemmer D, Zgurskaya H, Kustu S.
|
| Title |
Epistatic effects of the protease/chaperone HflB on some damaged forms of the Escherichia coli ammonium channel AmtB.
|
| Journal |
Genetics
|
| Abstract |
The Escherichia coli ammonium channel AmtB is a trimer in which each monomer carries a pore for substrate conduction and a cytoplasmic C-terminal extension of approximately 25 residues. Deletion of the entire extension leaves the protein with intermediate activity, but some smaller lesions in this region completely inactivate AmtB, as do some lesions in its cytoplasmic loops. We here provide genetic evidence that inactivation depends on the essential protease HflB, which appears to cause inactivation not as a protease but as a chaperone. Selection for restored function of AmtB is a positive selection for loss of the ATPase/chaperone activity of HflB and reveals that the conditional lethal phenotype for hflB is cold sensitivity. Deletion of only a few residues from the C terminus of damaged AmtB proteins seems to prevent HflB from acting on them. Either yields the intermediate activity of a complete C-terminal deletion. HflB apparently "tacks" damaged AmtB tails to the adjacent monomers. Knowing that HflB has intervened is prerequisite to determining the functional basis for AmtB inactivation.
|
| Volume |
183(4)
|
| Pages |
1327-40
|
| Published |
2009-12-1
|
| DOI |
10.1534/genetics.109.103747
|
| PII |
genetics.109.103747
|
| PMID |
19596908
|
| PMC |
PMC2787424
|
| MeSH |
ATP-Dependent Proteases / chemistry
ATP-Dependent Proteases / metabolism*
Amino Acid Sequence
Cation Transport Proteins / chemistry
Cation Transport Proteins / genetics*
Cation Transport Proteins / metabolism
Epistasis, Genetic*
Escherichia coli / genetics*
Escherichia coli / metabolism*
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / metabolism*
Models, Molecular
Molecular Chaperones / chemistry
Molecular Chaperones / metabolism*
Molecular Sequence Data
Mutant Proteins / chemistry
Mutant Proteins / genetics*
Mutant Proteins / metabolism
Protein Conformation
Suppression, Genetic
|
| IF |
4.015
|
| Times Cited |
10
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| Prokaryotes E. coli |
JW0441
JW3140
JW4133
JW4132
JW0442 |
