RRC ID 4965
Author Isak G, Rydén-Aulin M.
Title Hypomodification of the wobble base in tRNAGlu, tRNALys, and tRNAGln suppresses the temperature-sensitive phenotype caused by mutant release factor 1.
Journal J. Bacteriol.
Abstract In Escherichia coli, release factor 1 (RF1) is one of two RFs that mediate termination; it specifically recognizes UAA and UAG stop codons. A mutant allele, prfA1, coding for an RF1 that causes temperature-sensitive (Ts) growth at 42 degrees C, was used to select for temperature-resistant (Ts(+)) suppressors. This study describes one such suppressor that is the result of an IS10 insertion into the cysB gene, giving a Cys(-) phenotype. CysB is a transcription factor regulating the cys regulon, mainly as an activator, which explains the Cys(-) phenotype. We have found that suppression is a consequence of the lost ability to donate sulfur to enzymes involved in the synthesis of thiolated nucleosides. From genetic analyses we conclude that it is the lack of the 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) modification of the wobble base of tRNA(Glu), tRNA(Lys), and/or tRNA(Gln) that causes the suppressor phenotype.
Volume 191(5)
Pages 1604-9
Published 2009-3
DOI 10.1128/JB.01485-08
PII JB.01485-08
PMID 19103926
PMC PMC2648207
MeSH Alleles Bacterial Proteins / genetics* Bacterial Proteins / metabolism Codon, Terminator Culture Media DNA Transposable Elements Escherichia coli / genetics Escherichia coli / growth & development* Escherichia coli / physiology Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism Gene Expression Regulation, Bacterial Hot Temperature Peptide Termination Factors / genetics* Peptide Termination Factors / metabolism Phenotype RNA, Transfer, Gln* / chemistry RNA, Transfer, Gln* / genetics RNA, Transfer, Gln* / metabolism RNA, Transfer, Glu* / chemistry RNA, Transfer, Glu* / genetics RNA, Transfer, Glu* / metabolism RNA, Transfer, Lys* / chemistry RNA, Transfer, Lys* / genetics RNA, Transfer, Lys* / metabolism Suppression, Genetic* Temperature* Thiouridine / analogs & derivatives Thiouridine / metabolism
IF 3.219
Times Cited 3
Prokaryotes E. coli ME9062(BW25113) JW0413 JW0658 JW1267 JW3305 ME7986 (MG1655)