| RRC ID |
4965
|
| 著者 |
Isak G, Rydén-Aulin M.
|
| タイトル |
Hypomodification of the wobble base in tRNAGlu, tRNALys, and tRNAGln suppresses the temperature-sensitive phenotype caused by mutant release factor 1.
|
| ジャーナル |
J Bacteriol
|
| Abstract |
In Escherichia coli, release factor 1 (RF1) is one of two RFs that mediate termination; it specifically recognizes UAA and UAG stop codons. A mutant allele, prfA1, coding for an RF1 that causes temperature-sensitive (Ts) growth at 42 degrees C, was used to select for temperature-resistant (Ts(+)) suppressors. This study describes one such suppressor that is the result of an IS10 insertion into the cysB gene, giving a Cys(-) phenotype. CysB is a transcription factor regulating the cys regulon, mainly as an activator, which explains the Cys(-) phenotype. We have found that suppression is a consequence of the lost ability to donate sulfur to enzymes involved in the synthesis of thiolated nucleosides. From genetic analyses we conclude that it is the lack of the 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) modification of the wobble base of tRNA(Glu), tRNA(Lys), and/or tRNA(Gln) that causes the suppressor phenotype.
|
| 巻・号 |
191(5)
|
| ページ |
1604-9
|
| 公開日 |
2009-3-1
|
| DOI |
10.1128/JB.01485-08
|
| PII |
JB.01485-08
|
| PMID |
19103926
|
| PMC |
PMC2648207
|
| MeSH |
Alleles
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Codon, Terminator
Culture Media
DNA Transposable Elements
Escherichia coli / genetics
Escherichia coli / growth & development*
Escherichia coli / physiology
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / metabolism
Gene Expression Regulation, Bacterial
Hot Temperature
Peptide Termination Factors / genetics*
Peptide Termination Factors / metabolism
Phenotype
RNA, Transfer, Gln* / chemistry
RNA, Transfer, Gln* / genetics
RNA, Transfer, Gln* / metabolism
RNA, Transfer, Glu* / chemistry
RNA, Transfer, Glu* / genetics
RNA, Transfer, Glu* / metabolism
RNA, Transfer, Lys* / chemistry
RNA, Transfer, Lys* / genetics
RNA, Transfer, Lys* / metabolism
Suppression, Genetic*
Temperature*
Thiouridine / analogs & derivatives
Thiouridine / metabolism
|
| IF |
3.006
|
| 引用数 |
3
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
ME9062(BW25113)
JW0413
JW0658
JW1267
JW3305
ME7986 (MG1655) |
