論文 - 詳細
| RRC ID | 4965 |
|---|---|
| 著者 | Isak G, Rydén-Aulin M. |
| タイトル | Hypomodification of the wobble base in tRNAGlu, tRNALys, and tRNAGln suppresses the temperature-sensitive phenotype caused by mutant release factor 1. |
| ジャーナル | J Bacteriol |
| Abstract |
In Escherichia coli, release factor 1 (RF1) is one of two RFs that mediate termination; it specifically recognizes UAA and UAG stop codons. A mutant allele, prfA1, coding for an RF1 that causes temperature-sensitive (Ts) growth at 42 degrees C, was used to select for temperature-resistant (Ts(+)) suppressors. This study describes one such suppressor that is the result of an IS10 insertion into the cysB gene, giving a Cys(-) phenotype. CysB is a transcription factor regulating the cys regulon, mainly as an activator, which explains the Cys(-) phenotype. We have found that suppression is a consequence of the lost ability to donate sulfur to enzymes involved in the synthesis of thiolated nucleosides. From genetic analyses we conclude that it is the lack of the 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) modification of the wobble base of tRNA(Glu), tRNA(Lys), and/or tRNA(Gln) that causes the suppressor phenotype. |
| 巻・号 | 191(5) |
| ページ | 1604-9 |
| 公開日 | 2009-3-1 |
| DOI | 10.1128/JB.01485-08 |
| PII | JB.01485-08 |
| PMID | 19103926 |
| PMC | PMC2648207 |
| MeSH | Alleles Bacterial Proteins / genetics* Bacterial Proteins / metabolism Codon, Terminator Culture Media DNA Transposable Elements Escherichia coli / genetics Escherichia coli / growth & development* Escherichia coli / physiology Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism Gene Expression Regulation, Bacterial Hot Temperature Peptide Termination Factors / genetics* Peptide Termination Factors / metabolism Phenotype RNA, Transfer, Gln* / chemistry RNA, Transfer, Gln* / genetics RNA, Transfer, Gln* / metabolism RNA, Transfer, Glu* / chemistry RNA, Transfer, Glu* / genetics RNA, Transfer, Glu* / metabolism RNA, Transfer, Lys* / chemistry RNA, Transfer, Lys* / genetics RNA, Transfer, Lys* / metabolism Suppression, Genetic* Temperature* Thiouridine / analogs & derivatives Thiouridine / metabolism |
| IF | 3.006 |
| 引用数 | 3 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9062(BW25113) JW0413 JW0658 JW1267 JW3305 ME7986 (MG1655) |