RRC ID 4967
Author Kashiwagi A, Sakurai T, Tsuru S, Ying BW, Mori K, Yomo T.
Title Construction of Escherichia coli gene expression level perturbation collection.
Journal Metab Eng
Abstract We generated 61 strains of Escherichia coli in which the expression level of a specific single gene can be changed continuously over a physiologically significant range. In each strain, one auxotrophic gene was deleted from its original position and reinserted at a specific position on the chromosome under the control of the tetA promoter. Therefore, the level of expression of the target gene can be controlled easily by altering the concentrations of inducers, e.g., anhydrotetracycline and doxycycline, in the medium. Protein and mRNA levels and changes in proliferation rate were examined in some of the strains in our collection to determine the ability to control the level of target gene expression over a physiologically significant range. These strains will be useful for extracting omics data sets and for the construction of genome-scale mathematical models, because causality between perturbations in gene expression level and their consequences can be clearly determined.
Volume 11(1)
Pages 56-63
Published 2009-1-1
DOI 10.1016/j.ymben.2008.08.002
PII S1096-7176(08)00058-X
PMID 18790072
MeSH Cloning, Molecular Escherichia coli / genetics Escherichia coli / metabolism* Gene Expression Regulation, Bacterial Genes, Bacterial / physiology* Molecular Sequence Data Mutation Promoter Regions, Genetic
IF 7.263
Times Cited 26
Prokaryotes E. coli ME8569(DH1)