RRC ID 49695
Author Rebman JK, Kirchoff KE, Walsh GS.
Title Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons.
Journal PLoS One
Abstract Collective migration depends on cell-cell interactions between neighbors that contribute to their overall directionality, yet the mechanisms that control the coordinated migration of neurons remains to be elucidated. During hindbrain development, facial branchiomotor neurons (FBMNs) undergo a stereotypic tangential caudal migration from their place of birth in rhombomere (r)4 to their final location in r6/7. FBMNs engage in collective cell migration that depends on neuron-to-neuron interactions to facilitate caudal directionality. Here, we demonstrate that Cadherin-2-mediated neuron-to-neuron adhesion is necessary for directional and collective migration of FBMNs. We generated stable transgenic zebrafish expressing dominant-negative Cadherin-2 (Cdh2ΔEC) driven by the islet1 promoter. Cell-autonomous inactivation of Cadherin-2 function led to non-directional migration of FBMNs and a defect in caudal tangential migration. Additionally, mosaic analysis revealed that Cdh2ΔEC-expressing FBMNs are not influenced to migrate caudally by neighboring wild-type FBMNs due to a defect in collective cell migration. Taken together, our data suggest that Cadherin-2 plays an essential cell-autonomous role in mediating the collective migration of FBMNs.
Volume 11(10)
Pages e0164433
Published 2016-1-1
DOI 10.1371/journal.pone.0164433
PII PONE-D-16-21757
PMID 27716840
PMC PMC5055392
MeSH Adaptor Proteins, Signal Transducing / metabolism Animals Animals, Genetically Modified / metabolism Animals, Genetically Modified / physiology Cadherins / metabolism* Cell Adhesion / physiology Cell Movement / physiology* Cell Polarity / physiology Facial Nerve / physiology* Motor Neurons / metabolism* Motor Neurons / physiology* Promoter Regions, Genetic / physiology Rhombencephalon / embryology Rhombencephalon / metabolism Rhombencephalon / physiology Zebrafish Zebrafish Proteins / metabolism
IF 2.776
Times Cited 5
Zebrafish Tg(CM-isl1:GFP)