RRC ID 49699
Author Cayuso J, Dzementsei A, Fischer JC, Karemore G, Caviglia S, Bartholdson J, Wright GJ, Ober EA.
Title EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality.
Journal Dev Cell
Abstract Positioning organs in the body often requires the movement of multiple tissues, yet the molecular and cellular mechanisms coordinating such movements are largely unknown. Here, we show that bidirectional signaling between EphrinB1 and EphB3b coordinates the movements of the hepatic endoderm and adjacent lateral plate mesoderm (LPM), resulting in asymmetric positioning of the zebrafish liver. EphrinB1 in hepatoblasts regulates directional migration and mediates interactions with the LPM, where EphB3b controls polarity and movement of the LPM. EphB3b in the LPM concomitantly repels hepatoblasts to move leftward into the liver bud. Cellular protrusions controlled by Eph/Ephrin signaling mediate hepatoblast motility and long-distance cell-cell contacts with the LPM beyond immediate tissue interfaces. Mechanistically, intracellular EphrinB1 domains mediate EphB3b-independent hepatoblast extension formation, while EpB3b interactions cause their destabilization. We propose that bidirectional short- and long-distance cell interactions between epithelial and mesenchyme-like tissues coordinate liver bud formation and laterality via cell repulsion.
Volume 39(3)
Pages 316-328
Published 2016-11-7
DOI 10.1016/j.devcel.2016.10.009
PII S1534-5807(16)30722-5
PMID 27825440
PMC PMC5107609
MeSH Animals Body Patterning Cell Movement Cell Shape Ephrin-B1 / metabolism* Ephrin-B3 / metabolism* Epithelium / embryology* Epithelium / metabolism Functional Laterality* Liver / embryology* Mesoderm / embryology* Mesoderm / metabolism Morphogenesis* Pseudopodia / metabolism Receptors, Eph Family / metabolism* Zebrafish / embryology Zebrafish / metabolism Zebrafish Proteins / metabolism*
IF 10.092
Times Cited 19
Resource
Zebrafish Tg(sox17:EGFP)