RRC ID 49700
著者 Icha J, Kunath C, Rocha-Martins M, Norden C.
タイトル Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina.
ジャーナル J Cell Biol
Abstract The arrangement of neurons into distinct layers is critical for neuronal connectivity and function. During development, most neurons move from their birthplace to the appropriate layer, where they polarize. However, kinetics and modes of many neuronal translocation events still await exploration. In this study, we investigate retinal ganglion cell (RGC) translocation across the embryonic zebrafish retina. After completing their translocation, RGCs establish the most basal retinal layer where they form the optic nerve. Using in toto light sheet microscopy, we show that somal translocation of RGCs is a fast and directed event. It depends on basal process attachment and stabilized microtubules. Interestingly, interference with somal translocation induces a switch to multipolar migration. This multipolar mode is less efficient but still leads to successful RGC layer formation. When both modes are inhibited though, RGCs fail to translocate and induce lamination defects. This indicates that correct RGC translocation is crucial for subsequent retinal lamination.
巻・号 215(2)
ページ 259-275
公開日 2016-10-24
DOI 10.1083/jcb.201604095
PII jcb.201604095
PMID 27810916
PMC PMC5084647
MeSH Actin-Related Protein 2-3 Complex / metabolism Animals Cell Differentiation Cell Movement* Cell Nucleus / metabolism Cell Survival Embryo, Nonmammalian / cytology Kinetics Microtubules / metabolism Models, Biological Organelles / metabolism Retinal Ganglion Cells / cytology* Stem Cells / cytology Stem Cells / metabolism Zebrafish / embryology Zebrafish / metabolism*
IF 8.811
引用数 24
リソース情報
ゼブラフィッシュ Tg(vsx1:EGFP)